转化生长因子β_1、胰岛素样生长因子1受体在子宫肌瘤中的意义  

Expression of transforming growth factor beta-1 and insulin like growth factor-1 receptor in human uterine leiomyoma

作　　者：孙建清[1] 郭美君[2] 连瑜[2] 

机构地区：[1]包头市中心医院妇产科,内蒙古包头014040 [2]内蒙古医学院,内蒙古呼和浩特010059

出　　处：《实用肿瘤杂志》2008年第4期322-325,共4页Journal of Practical Oncology

摘　　要：目的探讨转化生长因子β1(TGF-β1)和胰岛素样生长因子1受体(IGF-1R)在子宫肌瘤中的表达及其相关性。方法采用原位杂交方法检测50例子宫肌瘤及瘤旁正常的子宫肌层中的TGFβ1 mRNA的表达,同时采用免疫组化技术检测相应标本中TGFβ1和IGF-1R蛋白的表达。结果(1)TGFβ1 mRNA及蛋白阳性表达均位于细胞浆。在子宫肌瘤中的表达水平分别为1.02±0.38、1.42±0.67,在相应的正常子宫肌壁中的表达水平分别为0.66±0.48、0.58±0.50,两者差异有统计学意义(P<0.01)。(2)IGF-1R在子宫肌瘤和相应的子宫肌壁中的表达水平分别为1.92±0.88、0.80±0.49,两者比较差异有统计学意义(P<0.01)。(3)各指标在增殖期、分泌期肌瘤和肌壁中的表达差异无统计学意义。(4)TGFβ1与IGF-1R无相关性。结论TGFβ1、IGF-1R是子宫肌瘤生长的重要因素。Objective To investigate the expression of transforming growth factor beta-1 （TGF-β1）and insulin like growth factor-1 receptor （IGF-1R） in human uterine leiomyoma and myometrium. Methods Fifty surgical specimens of uterine leiomyoma and corresponding normal myometrium were collected. The expression of TGF-β1 mRNA was detected by in situ hybridization （ISH）; while the expression of TGF-β1 and IGF-1R proteins was detected by immunohistochemical S-P method. Results The positive expression of TGF-β1 mRNA and protein were all located in cytoplasm. The expressive values of TGF-β1 mRNA and protein in uterine leiomyoma were 1.02±0. 38 and 1.42±0.67, respectively,while those in matched normal myometrium were 0.66±0.48 and 0.58±0.50, respectively （P〈0.01）. The expressive values of IGF-1R in human uterine leiomyoma and normal myometrium were 1.92±0.88 and 0.80±0.49, respectively （P〈0.01）. There were no significant differences in the expression of TGF-β1 mRNA and protein,IGF-1R protein between the proliferative and secretory phases both in uterine leiomyoma and normal myometrium. The expression of TGF-β1 protein was not correlated with that of IGF-1R. Conclusions TGFβ1 and IGF-1R might he important factors for growth of uterine leiomyoma.

关 键 词：子宫肿瘤/病理学 平滑肌瘤/病理学 转化生长因子Β 受体 IGF1型 原位杂交 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]