束缚性应激对大鼠血小板NO合成通路的影响  

Effect of water-immersion restraint stress on nitric oxide production pathway in rat platelets

作　　者：冯浩楼[1] 崔玉英[2] 魏芳[2] 王保水[2] 唐朝枢[3] 

机构地区：[1]河北大学医学部,河北保定071000 [2]河北大学卫生职业技术学院生理教研室,河北保定071000 [3]北京大学第一医院心血管研究所,北京100034

出　　处：《基础医学与临床》2008年第7期719-722,共4页Basic and Clinical Medicine

基　　金：河北省科学技术研究与发展计划项目(06276121);保定市科技攻关课题(06F04-1)

摘　　要：目的观察急性应激对大鼠血小板一氧化氮(NO)释放的影响及其机制。方法大鼠浸水-束缚应激(WRS)2、4和8 h,以胃溃疡指数(UI)作为应激损伤的标识,采用Greiss法测定血小板孵育液中亚硝酸盐(NO2-)含量;同位素法测其一氧化氮合酶(NOS)活性和L-精氨酸(L-Arg)转运量。结果WRS 2 h血小板L-Arg转运量、NOS活性和孵育液NO2-含量较对照组显著增加,但随应激时间延长,其呈下降趋势,应激8 h时均显著低于对照组,胃溃疡逐渐加重。结论WRS应激早期阶段可上调血小板L-Arg/NO通路,促进血小板NO生成;长时间应激下调L-Arg/NO通路,减少NO释放。Objective To investigate the effect of water-immersion restraint stress （WRS） on nitric oxide （NO） production and its mechanism in rat platelets. Methods Male SD rats underwent WIR stress for 2, 4 and 8 hours. Ulcer index （UI） of stomach was chedsed by Guth method, nitrite production was measured by Greiss assay, nitric oxide synthase （NOS） activities and L-arginine transport rate were determined by isotope tracer method. Results After 2 h WRS, the NO production, the NOS activity and L-arginine transport rate in rat platelets obviously increased and then gradually decreased. After 8 h of WRS, these parameters were decreased as compared with the control group. The gastric ulcer appeared and got more serious after 4 h WIR stress. Conclusion Short-term WRS up-regulates L-arginine/NO pathway and increases nitric oxide production in the platelets but long-term WIR stress has the reversed effect.

关 键 词：束缚应激 血小板 一氧化氮 一氧化氮合酶 L-精氨酸转运 

分 类 号：R363.2[医药卫生—病理学] R33[医药卫生—基础医学]