金粉蕨素体外抗人卵巢癌作用研究  

Antitumor Effect of Onychin on Ovarian Cancer Cell in Vitro

作　　者：唐华贵 谢宛玉 曹建国[2] 刘艳娥 

机构地区：[1]深圳市宝安区福永医院,深圳市518103 [2]南华大学肿瘤研究所,衡州市421001

出　　处：《医药论坛杂志》2008年第15期4-6,共3页Journal of Medical Forum

摘　　要：目的探讨金粉蕨素抗人卵巢癌(COC1)细胞系细胞作用及机制。方法MTT比色法测定金粉蕨素对COC1、顺铂耐药亚株COC1/DDP及中国仓鼠卵巢(CHO-K1)细胞系细胞增殖的影响;PI染色流式细胞术(FCM)分析金粉蕨素对COC1、顺铂耐药亚株COC1/DDP及中国仓鼠卵巢(CHO-K1)细胞系细胞凋亡和细胞周期的影响。结果金粉蕨素对COC1、COC1/DDP细胞生长具有抑制作用呈剂量-效应和时间-效应关系,PI染色FCM分析发现金粉蕨素明显使COC1细胞周期停滞于G1期(G1期细胞百分数由50.3%上升到70.6%,P<0.05)。结论金粉蕨素具有体外抗人卵巢癌(COC1)细胞系细胞作用,其机制可能与细胞周期G1期阻滞有关。Objective To study the effects and mechanism of the onychin on human ovarian cancer（COC1 ） cello. Methods MTT assay was used to determine the effect on proliferation of COC1 , COC1/ddp and CHO -K1 cell by onychin;PI stained flow eytometry （FCM） was used to analyse the apoptosis and cell eyele change of COC1 treated by Onyehin. Results Onyehin inhibited COC1 and COC1/DDP cell growth signifieantly in a dose - dependent manner and a time - dependent manner. Analysis by flow cytometry showed that onychin suppressed cell cycle progression at Gi phase. 9 （ the percentages of cells iu G1 phase rose from 50. 3% to 70.6% ）. Conclusion Onyehin has anti - ovarian carcinoma effect in vitro; the mechanism might be associated with G1 phase cell cycle arrest.

关 键 词：卵巢癌 金粉蕨素 治疗作用 

分 类 号：R737.31[医药卫生—肿瘤]