中药添加剂对高温下猪血清抗氧化功能的影响  

Effect of traditional Chinese medicine additives on serum antioxidant status in piglets under high temperature

作　　者：宋小珍[1] 王占赫[2] 毛帅[2] 刘凤华[2] 王恬[1] 

机构地区：[1]南京农业大学动物科学院 [2]北京农学院动物科技系,北京102206

出　　处：《畜牧与兽医》2008年第5期5-8,共4页Animal Husbandry & Veterinary Medicine

基　　金：北京市科委攻关课题(Y0705003041631);国家自然科学基金(30771566,30771591);北京市教委资助重点课题(KZ200310020007)

摘　　要：为探讨中药添加剂对高温应激仔猪抗氧化功能的调控效果,选用35日龄中国实验用小型猪64头,分为对照组、应激组和2个中药添加剂组(方1、方2),分别于试验第1,2,4,6,10天采血清,测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的含量变化。结果发现:与对照组相比,应激组猪血清SOD活力在第1天显著升高;MDA含量在第6天显著升高;GSH-Px活力在第6和10天显著高于常温组(P<0.05)。与应激组相比,方2组的GSH-Px活力在第2天显著升高,方1和方2组MDA含量在第10天显著降低(P<0.05)。表明高温状态下,猪体内MDA含量增加,GSH-Px活性增强;中药添加剂能促进热应激仔猪抗氧化功能的恢复和提高,且方2效果优于方1。In order to determine the effect of traditional Chinese medicine additives on serum antioxidant status in piglets under high temperature, 64 weaned piglets of 35 days-old with an initial weight of 6. 50 ± 1.05 kg were randomly divided into four groups ： Normal temperature control group, NTC; High temperature stress group, HTS; Chinese medicine additives group Ⅰ （CMA Ⅰ）, and Chinese medicine additives group Ⅱ （ CMA Ⅱ ）. In each group, 4 piglets were collected for blood samples after treatment on day 1,2, 4, 6 and 10, respectively. Serum activity of SOD and GSH-Px and MDA content were determined. The result showed ： 1 ） Serum SOD activity was increased at day 1, MDA content increased at day 6, and GSH-Px activity increased at days 6 and 10 in HTS group compared with the NTC group （P 〈 0. 05） ; 2） MDA content was decreased at day 10 in CMA Ⅰ and CMA Ⅱ group, but GSH-Px activity was increased significantly at day 2 in CMA Ⅱ group compared with the HTS group （ P 〈 0.05 ）. The experiment results also indicated that the content of MDA was increased and the serum activity of GSH-Px was increased under high-temperature stress. Our results suggested that traditional Chinese medicine additives play a important role on adjusting serum antioxidant status in piglets under high-temperature stress ; In addition, CMA Ⅱ is better than CMAI.

关 键 词：中药添加剂 热应激 猪 抗氧化功能 

分 类 号：S816.7[农业科学—饲料科学]