PPAR-γ的激动剂吡咯列酮抑制肝癌细胞生长和诱导凋亡的实验研究  

The effects of Pioglitazone,a Peroxisome proliferator-activated receptor-gamma ligand on antiproliferation and induce apoptosis in human hepatocellular carcinoma cell lines SMMC7721

作　　者：丁斌[1] 谢勇[2] 周南进[3] 孙辉[1] 

机构地区：[1]江西省九江市第一人民医院消化内科,九江332000 [2]南昌大学第一附属医院消化研究所 [3]江西省医学科学研究所,南昌330006

出　　处：《江西医药》2008年第7期654-656,共3页Jiangxi Medical Journal

摘　　要：目的观察PPAR-γ的激动剂吡咯列酮对肝癌细胞的生长抑制作用和诱导凋亡作用。方法吡咯列酮作用于肝癌细胞株SMMC7721,MTT法检测肝癌细胞的生长抑制率;流式细胞仪检测肝癌细胞凋亡率;RT-PCR检测肝癌细胞内PPAR-γmRNA的表达。结果吡咯列酮对肝癌细胞有明显抑制作用和诱导凋亡作用;细胞凋亡率与细胞生长抑制率之间无显著相关性(r=0.639,P=0.361);肝癌细胞SMMC7721表达PPAR-γmRNA,随吡咯列酮浓度的增加肝癌细胞内PPAR-γmRNA的表达呈下降趋势。结论PPAR-γ的激动剂吡咯列酮可抑制肝癌细胞的生长和诱导其凋亡;但是,细胞凋亡率与生长抑制率之间无显著相关性,提示尚有诱导细胞凋亡以外的途径参与吡咯列酮对肝癌细胞的生长抑制作用;吡咯列酮可下调肝癌细胞内PPAR-γmRNA的转录,具体机制有待研究。Objective. To study the effect of Pioglitazone,a Ligand of Peroxisome proliferator-activated receptor-gamma,for Antiproliferation and induction of apoptosis in hepatocellular carcinoma （HCC） cell lines（SMCC7721）.Methods HCC line SMMC7721 was treated with Pioglitazone.Antiproliferation effects was measured by MTT method.The rate of apoptosis was detected by flow cytometry. The expression of PPAR-γ was detected by RT-PCR.Results Pioglitazone inhibited the growth of SMMC7721 and induced it to apoptosis, the rate of apoptosis and the rate of inhibition of the growth of cells were not significantly （r=0.639 ,P=0.361 ）.Hepatocarcinoma cells SMMC7721 expressed PPAR-γmRNA.And the expression of PPAR-γmRNA in hepatocarcinoma cells had the descending tendency after Pioglitazone acted on hepatocarcinoma cells which was enhanced when the concentration of Pioglitazone was increased.Conclusion Pioglitazone could inhibit the growth of HCC cell line in vitro and induce the apoptosis of hepatocarcinoma cells. But the rate of apoptosis and the rate of inhibition of growth were not significantly relativity, which pointed out that yet there was other way participating in the effect of inhibition on the growth of hepatocarcinoma cells other than apoptosis.Pioglitazone could down regulate the expression of PPAR-γ/mRNA in cells,but the specific mechanism is not clear, which needs further investigation.

关 键 词：盐酸吡咯列酮 过氧化物酶体增生物激活受体-Γ 凋亡 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]