出 处:《中华中医药学刊》2008年第8期1684-1687,共4页Chinese Archives of Traditional Chinese Medicine
基 金:国家自然科学基金资助项目(30672570)
摘 要:目的:通过研究摘除卵巢致肾虚骨质疏松症大鼠肾组织中Smurf2的mRNA和蛋白的表达,揭示摘除卵巢致肾虚骨质疏松症的病理机制,并研究补肾中药防治疗效及其作用机制。方法:摘除雌性大鼠双侧卵巢的方法复制肾虚骨质疏松症模型,采用具有补肾壮骨益精作用的补肾中药(高、低剂量)对实验大鼠治疗12周,以骨疏康颗粒剂、盖天力片作为阳性对照组,正常大鼠、假手术组作为标准对照组,模型大鼠作为空白对照组,并与补脾中药作疗效的比较。用RT-PCR法、Western印迹法检测肾虚骨质疏松症大鼠肾组织中Smurf2的mRNA和蛋白表达。结果:RT-PCR及Western方法检测,正常大鼠肾组织中存在Smurf2的mRNA和蛋白表达;与正常组、假手术组比较,模空组Smurf2 mRNA和蛋白的表达水平在肾组织中明显降低。用药12周后,与模空组比较,补肾高、低剂量组、盖天力组可明显上调Smurf2 mRNA和蛋白在肾组织中的表达水平。其中补肾高剂量组上调幅度大于补肾低剂量组。结论:①正常大鼠肾组织中Smurf2可以在基因、蛋白水平表达;②骨质疏松症的发生,可能与肾组织中Smurf2的mRNA和蛋白表达的异常变化有关;③补肾中药通过调控肾组织中Smurf2的mRNA和蛋白表达,对于骨质疏松症有一定的防治作用。Objective : In order to explore the etiological factors and pathologh of the osteoporosis due to kidney deficiency,We study the expression of smurf2 mRNA and proteins in the kidney of the rats of osteoporosis due to kidney deficiency by using ovariectomy. To explore the adjustable function of Chinese medicine and its regulating mechanism. Methods:By using ovariectomy, we established the rat model of osteoporosis due to Kidney-deficiency. We treated the osteopomsis rat with tonifying kidney Chinese herbs (high dose, small dosage) for 12 weeks. Simultaneously,used GuShuKang granule and GaiTianLi tablets, as positive control groups. Used tonifying spleen Chinese herbs, as curative effeetion group. And rats in the normal group and model group were not given any treatment. We used the RT- PCR and Western Blotting method to investigate the expression of smurf2 mPNA and proteins in the kidney of the rats. Results : RT - PCR and West- ern blotting detection:the Smurf2 existed in the normal kidney tissue. Comparing with the normal group and fake operational group,the level of the expression of the gene and protein of Smurf2 in the kidney tissue of model group decreased evidently. After the precaution treatment with the experimental drug groups (high, small dosage), Gaitianli tablets group can evidently increase the mRNA and protein expression level of Smurf2 in the kidney tissue. The experimental drug group ( (high dosage) is the better than the small dosage group. Conclusion :①In normal rats,the tissue of the kidney can express the smurt2 in the gene and protein level.②The changing of smurf2 mRNA and protein expression in the kidney tissue,may be one of the important occurring mechanism of the osteoporosis due to kidney deficiency. ③The experimental Chinese herbs, which have the function of tonifying kidney, can increase the level of the expression of the gene and protein of smurf2 in the kidney tissue. It would be preventing and treating to the osteoporosis due to kidney deficiency.
关 键 词:骨质疏松症 肾虚 Smad泛素调节因子2
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