植物组织培养的三大难题  

Three Difficult Problems in Plant Tissue Culture

作　　者：杨丽琴[1] 李瑞[1] 王俊[1] 胡海英[1] 吴晓玲[1] 

机构地区：[1]宁夏大学,宁夏银川750021

出　　处：《北方园艺》2008年第4期104-107,共4页Northern Horticulture

基　　金：国家科技攻关资助项目(2005BA901A18)

摘　　要：在植物组织培养过程中,常发生褐变、污染、玻璃化现象三大难题,严重影响了组织培养材料的正常发育,给组织培养带来了一定的困难。结合近些年的研究,对三者发生的原因以及控制措施进行了综述,以期为科研和生产提供一定的理论依据和实践指导。There are three difficult problems in plant tissue culture： browning, contamination and vitrification. These badly affect the normal development of tissue culture material, and influence the proceedings of tissue culture. Combined with the recent years research, this paper analyzed the reason why these phenomenons come out and poses some suggestions on how to control them, which can provide theory and practice evidence for science research and production.

关 键 词：组织培养 褐变 污染 玻璃化 

分 类 号：Q943.1[生物学—植物学]