铜对大蒜根尖细胞有丝分裂影响的研究  

The Study of the Effect of Copper on the Caryomitosis of Garlic Root Tips

作　　者：高扬[1] 辛树权[1] 何锦冰[1] 侯伟[1] 

机构地区：[1]长春师范学院生命科学学院,吉林长春130032

出　　处：《北方园艺》2008年第6期12-14,共3页Northern Horticulture

摘　　要：用不同浓度(0.05、0.10、0.25、0.50、1.00g/L)的硫酸铜(Cu2+)溶液作为诱变剂,分别处理大蒜根尖6、18、24h。通过常规染色体压片技术,观察大蒜根尖细胞有丝分裂现象。结果表明:不同浓度的硫酸铜溶液均能使大蒜根尖细胞有丝分裂指数明显下降。其中染毒18h、Cu2+的浓度为0.10、0.25g/L时,有丝分裂指数明显小于其他浓度;染毒24h、Cu2+浓度为0.50、1.00g/L时,有丝分裂指数明显小于其他浓度;而染毒24h、Cu2+浓度为0.05g/L时,有丝分裂指数抑制作用最小。同时还发现:不同时间不同浓度的硫酸铜溶液均能导致多种类型的染色体畸变,且畸变率明显高于对照组。结论:硫酸铜对大蒜根尖细胞具有明显的细胞毒性和遗传毒性。Using different concentrations（0. 05,0. 10,0. 25,0. 50,1.00 g/L） of CuSO4 as mutagenic substance, we treated the garlic root tips for 6 h, 18 h and 24 h, respectively. And then observed the phenomenon of its caryomitosis vdth common technique of chromosome^tabletting. The result indicated that all concentrations of it significantly decreased the Chromosome index. Among them, both the concentrations of Cu^2＋ were 0.10 g/L and 0. 25 g/L whose chromosome in dex by far less than others when treated for 18 h; however, when the tin,e-treated was up to 24 h, 0. 50 g/I. and 1.00 g/L of CuSO4 gave rise to a significantly less caryomitosis index and the inhibitory effect of CuSO4 on the chromosome was least with 0. 05 g/L. Simultaneously, different time and concentrations-treatment could result in various chromo some aberrations, and had a higher aberration rate than control. We concluded that CuSO4 rendered the cells of garlic root tip more cyto-toxicity and inherent toxicity.

关 键 词：硫酸铜 大蒜 有丝分裂指数 染色体畸变 

分 类 号：S633.404.1[农业科学—蔬菜学]