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出 处:《北方园艺》2008年第8期178-180,共3页Northern Horticulture
基 金:国家自然科学基金资助项目(306771242);聊城大学重点科研基金资助项目(X061006)
摘 要:采用聚丙烯酰胺凝胶电泳,研究了黄桃新品种‘黄金冠’与‘锦绣’桃过氧化物酶同工酶谱的数量和活性相对含量,结果表明:‘黄金冠’与‘锦绣’的过氧化物酶同工酶谱酶带数量相同,级别相似。与其它品种比较,不同器官间酶带的数量、级别、活性相对含量都存在着较为明显的差异,说明黄金冠和锦绣的同源性强,亲缘关系较近。同时,‘黄金冠’与‘锦绣’在过氧化物酶同工酶酶谱上,酶活性有差异,证明黄金冠已不同于锦绣,在遗传上发生了变异。该研究对于桃品种分类、选育及生产应用等具有一定的参考价值和借鉴意义。This article adopted polypropylene aromatic gel electrophoresis to study the number of peroxide enzyme spectrum and the relative content of activity of the new variety of Huangjinguan and Jinxiutao. The results showed that the enzyme band number of peroxide enzyme spectrum was same and the rank was similar between Huangjinguan and Jinxiutao. Compared with the other variety, there exited relatively differences between the number, rank and reactive's relative content of different organs. It explained that the homologous nature was strong and the affinity connection was close. At the same time, the enzyme active was different on peroxide enzyme spectrum of Huangjinguan and Jinxiu. It proved that Huangjinguan was now different from Jinxiu. It had become different during development. The study had certain consulted value and use for reference for the classification, breeding and manufacturing or use of peach variety.
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