香兰素衍生物对辐射损伤细胞保护作用的初步研究  

THE PRIMARY STUDY ON PROTECTIVE EFFECTS OF VALLININ DERIVATIVE ON CELL INJURY INDUCED BY RADIATION

作　　者：郑红[1] 汪思应[1] 严雨倩[2] 王林[2] 徐勤枝[2] 丛建波[2] 周平坤[2] 

机构地区：[1]安徽医科大学病理生理教研室,合肥230032 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射防护》2008年第4期226-231,共6页Radiation Protection

基　　金：安徽省教育厅自然科学基金(2006KJ368B);安徽医科大学博士基金(XJ2006006)

摘　　要：采用四甲基偶氮唑盐微量酶反应比色方法（Methyl Thiazolyl Tetrazolium Colorimetric Assay．ATT法）和电子自旋共振法（ESR）初步探讨香兰素衍生物VND3207对辐射损伤细胞的保护作用。首先采用MTT方法进行香兰素衍生物VND3202-VND3209对人成纤维母细胞（HFS）的毒性检测，然后再通过MTT方法检测各衍生物对2Gy照射的人宫颈癌HeLa细胞增殖的影响及VND3207对4Gy照射的正常T淋巴母细胞系AHH-1细胞的影响；并通过电子自旋共振法（ESR）检测香兰素和VND3207的抗氧化活性。结果表明，8种衍生物单独作用HFS细胞72h，VND3206和VND3207在50μmol／L浓度范围内对正常细胞未见明显毒性；单独作用HeLa细胞72h后，VND3206在30μmol／L时对HeLa细胞有增殖作用（P〈0．05），而VND3207、VND3209对其没有明显的增殖效果，相反VND3208对HeLa细胞具有增敏作用；VND3207对照射后AHH-1细胞损伤具有保护作用；ESR进一步检测表明VND3207能够清除自由基，且清除能力大于香兰素（P〈0．05或P〈0．01）。本实验初步筛选出香兰素衍生物VND3207对辐射损伤细胞可能具有保护作用，为下一步研究工作提供了资料。In this paper, the protective effects of vallinin derivative VND3207 on cell injury induced by radiation were studied by the methods of methyl thiazolyl tetmzolitun colorimetric assay （MTT） and electron spin resonance （ESR）. At first, MTT method was used to evaluate the cytotoxicity of vallinin derivatives （VND3202 - VND3209） in HIS cells. Then, MTT method was used to measure the proliferation activity of HeLa cells with 2 Gy irradiation treated with vallinin derivatives and measure the proliferation of AHH-1 cells treated with VND3207 before exposed to 4 Gy irradiation. And ESR detected the antioxidation activity of vallinin and VND3207. The results showed that VND3207 and VND3206 presented no toxin within 50 panol/L, and VND3207 and VND3209 had no proliferous effects on HeLa cells while VND3206 could expedite the tumor cell proliferation at 30 μmol/L, and by contraries VND3208 showed increased radiosensitivity of the HeLa cells. For the AHH1 cells exposed to 4 Gy irradiation, VND3207 presented the protective effects against radiation injury. ESR results also suggested that VND3207 could clean out free radicals. Its effect was far more potent than that of vanillin. From this study we primarily screened out the vallinin derivative VND3207 which has protective effects on cell injury induced by radiation and provided data for future research work.

关 键 词：香兰素 香兰素衍生物 ^60CO γ射线细胞保护作用 

分 类 号：R285[医药卫生—中药学]