非小细胞肺癌血管内皮生长因子C表达的研究  

Expression of VEGF-C in Non-small Cell Lung Cancer

作　　者：杨华平[1] 吴鄂生[1] 陈清兰[1] 胡成平[1] 

机构地区：[1]中南大学湘雅医院呼吸内科,湖南长沙410008

出　　处：《医学临床研究》2008年第8期1423-1425,共3页Journal of Clinical Research

摘　　要：【目的】研究血管内皮生长因子C(VEGF-C)在非小细胞肺癌(NSCLC)中的表达及其与血管新生和淋巴结转移的关系。【方法】采用免疫组化方法检测40例NSCLC癌组织及10例正常肺支气管黏膜组织VEGF-C的表达,同时检测癌组织CD34、p53及p16基因的表达。【结果】40例NSCLC癌组织VEGF-C阳性表达率为77.8%,显著高于正常肺组织(20%,P<0.01)。40例肺癌组织中,VEGF-C高表达组平均微血管密度(MVD)显著高于其低表达组(P<0.01);有淋巴结转移者VEGF-C表达强度显著高于无淋巴结转移者(P<0.01);吸烟者的VEGF-C表达强度显著高于不吸烟者(P<0.05);p53阳性者VEGF-C表达强度显著高于其阴性者(P<0.01);p16阳性组与阴性组间VEGF-C表达强度比较差异无显著性(P>0.05)。【结论】VEGF-C的表达增高与NSCLC的血管新生程度及其淋巴结转移的形成密切相关,因而在NSCLC的生长、侵袭和淋巴结转移中起重要作用。NSCLC的VEGF-C过表达与抑癌基因p53的突变失活有关,而与p16无关。吸烟可能也是诱导VEGF-C表达上调的因素之一。[Objective]To investigate the expression of VEGF-C in non-small cell lung cancer （NSCLC） and its relationship with tumor angiogenesis and lymph node metastasis. [Methods]Forty tumour specimens resected from patients with NSCLC and 10 normal lung and bronchial mucosa tissues were investigated. The expression of VEGF-C was detected by an immunohistochemical method. Meanwhile the expressions of CD34, p53 and p16 in tumor tissues were also detected, [Results] The positive rate of VEGF-C in NSCLC was 77. 8% which was significantly higher than that in normal lung tissues （20%, P 〈0.01）. The mean microvessel density （MVD） was significantly higher in patients with high levels of VEGF-C expression than that in those with low levels of VEGF-C expression （ P 〈0.01）. VEGF-C expression was significantly higher in patients with lymph node metastasis than in those without lymph node metastasis （ P 〈0. 01）, and higher in patients with smoking than in those without smoking（ P 〈0. 05）, and higher in patients with p53-positive than in those with p53-negative（ P 〈0. 01）. There was no correlation between VEGF-C expression and p16 expression（ P〉0.05）. [Conclusion] There is very close correlation between VEGF-C expression and angiogenesis, lymph node metastasis and p53 expression in NSCLC. Smoking maybe causes the overexpression of VEGF-C in NSCLC.

关 键 词：癌 非小细胞肺 内皮生长因子 

分 类 号：R730.26[医药卫生—肿瘤]