皂荚提取物对人食管鳞癌细胞EC9706的生长抑制作用研究  

Effect of Chinese Honeylocust Fruit Extract on Proliferation of Esophageal Squamous Carcinoma Cell Line EC9706

作　　者：陈晓杰[1] 薛乐勋[1] 杨胜利[1] 

机构地区：[1]郑州大学第一附属医院食管癌研究室,河南郑州450052

出　　处：《癌变．畸变．突变》2008年第4期272-274,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家"十五""211工程"资助课题(教重办2002-2)

摘　　要：背景与目的：探讨皂荚提取物对人食管鳞癌细胞株EC9706的生长抑制作用及其作用机制。材料与方法：用皂荚提取物作用E9706细胞后，采用光学显微镜观察细胞形态学变化，用MTT法检测不同浓度皂荚提取物对EC9706细胞增殖的影响；采用TUNEL法检测皂荚提取物对EC9706细胞凋亡的影响；采用Westernblotting检测皂荚提取物对EC9706细胞bcl-2表达的影响。结果：不同浓度皂荚提取物（100—200μg／m1）对EC9706细胞的增殖有明显抑制作用（P〈0．01），并具明显的浓度依赖效应（r=0．768）；皂荚提取物可诱导EC9706细胞凋亡，抑制EC9706细胞bcl-2蛋白表达。结论：皂荚提取物可抑制EC9706细胞的增殖，诱导细胞凋亡。该效应可能与皂荚提取物抑制细胞bcl-2的表达有关。BACKGROUND AND AIM： This study was designed to observe the growth inhibition of the chinese honeylocust fruit extract on esophageal squamous carcinoma cell line EC9706. MATERIALS AND METHODS： The changes of cell morphology were examined by light microscope MTT assay , TUNEL cell apoptosis detection and western blotting were used to get information of EC9706 cells treated with the extract. RESULTS： Different concentrations （100-200 μg /ml）of the chinese honeylocust fruit extract significantly inhibited the growth of EC9706 cells（P 〈 0.01）,and displayed a concentration-dependent effect（r = 0.768） .The extract could induce apoptosis and regulate the expression of bcl-2 protein in EC9706 cells. CONCLUSION： The extract from chinese honeylocust fruit could inhibit the growth and induce the apoptosis of EC9706 cells. These may be associated with the decreased expression of bcl-2 in EC9706.

关 键 词：皂荚 MTT 凋亡 BCL-2 蛋白免疫印迹 

分 类 号：R730.45[医药卫生—肿瘤]