肿瘤相关基因chp2在白血病细胞中表达的研究  

Expression of A Tumor Related Gene chp2 in Leukemia Cells

作　　者：李彬 李洪强 马丽 芦颖 李庆华 茹永新 庞天翔 

机构地区：[1]中国医学科学院、北京协和医学院血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2008年第4期734-737,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号30570358);天津市自然科学基金项目(编号08JCZDJC19100)

摘　　要：为了探讨人类肿瘤相关基因chp2在白血病原代细胞和白血病细胞系中表达的特点,选择24例白血病患者、4种白血病细胞系及10名正常人外周血单个核细胞(PBMNC),用实时定量PCR(RQ-PCR)方法检测chp2基因的表达水平。结果显示,10例正常对照细胞chp2mRNA的表达检出率为80%,阳性的表达量为(0.744±0.682)×105cpsμ/。l白血病原代细胞和白血病细胞系chp2mRNA的表达量较正常对照组明显增高(p<0.05),原代细胞中的7例急性髓细胞白血病(AML)、6例慢性髓细胞白血病(CML)、7例急性淋巴细胞白血病(ALL)和4例慢性淋巴细胞白血病(CLL)的表达量分别是(11.637±5.588)、(6.122±3.785)、(4.262±2.561)和(3.434±1.974)×105cpsμ/l;白血病细胞系中K562细胞、Jurkat细胞、HL-60细胞和M07e细胞的表达量为(5.243±1.852)、(4.463±1.621)、(4.137±1.837)和(2.578±1.137)×106cpsμ/l,白血病细胞系的表达量高于原代细胞。结论:人类肿瘤相关基因chp2在白血病原代细胞和白血病细胞系中表达水平明显增高,提示其在白血病细胞生长过程中发挥着重要的作用。The purpose of this study was to investigate the expression feature of a human tumor related gene chp2 in leukemia primary cells and leukemia cell lines, real-time quantitative PCR （RQ-PCR）was performed to detect the expression level of chp2 gene in peripheral blood mononuclear cells from 10 healthy individuals（as control） and 24 cases of leukemia, and in 4 kinds of leukemia cell lines. The results showed that the detection rate of chp2 gene in 10 normal controls was 80%, positive expression was （0. 744 ±0. 682） × 10^5 cps/μl. The expression levels of chp2 mRNA leukemia primary cells and leukemia cell lines were significantly higher than that in the normal control （p 〈 0.05 ）. The expression levels of chp2 mRNA were higher in AML cells （7 cases）, CML cells （6 cases）, ALL cells （7 cases） and CLL cells （4 cases）, and their expression levels were （ 11. 637 ± 5. 588）, （6. 122 ± 3. 785）, （4.262 ± 2.561 ） and （3.434 ± 1.974） × 10^5 cps/μl respectively. Gene chp2 positively expressed in four kinds of leukemia cell lines, and the expression levels in K562 cells, Jurkat cells, HL-60 cells and M07e cells were （5. 243± 1. 852 ）, （4. 463 ± 1. 621 ）, （4. 137 ± 1. 837） and （2.578 ± 1. 137） × 10^6 cps/μl respectively. The expression level in leukemia cell lines was higher than that in primary cells. It is concluded that the human tumor related gene chp2 expression in leukemia primary cells and leukemia cell lines significantly increase, which may play an important role in growth process of leukemia cells.

关 键 词：chp2 肿瘤相关基因 白血病细胞 实时定量PCR 

分 类 号：R733.7[医药卫生—肿瘤]