Genistein对TGF-β1诱导人胰腺癌细胞上皮-间质样转化的作用  

Effect of Genistein on epithelial-mesenchymal transdifferentiation induced by transforming growth factor-β1 in human pancreatic cancer cell line Panc-1

作　　者：韩磊[1] 郭克建[1] 周文平[2] 陈希涛[1] 

机构地区：[1]中国医科大学附属第一医院普通外科,辽宁省沈阳市110001 [2]中国人民解放军沈阳军区总医院肝胆外科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2008年第21期2359-2363,共5页World Chinese Journal of Digestology

摘　　要：目的:研究Genistein对人胰腺癌细胞系Panc-1诱导细胞上皮-间质样转化的作用,以探讨Genistein抑制胰腺癌侵袭作用的机制.方法:用TGF-β1和不同浓度Genistein(0、1、25、50μmol/L)处理Panc-1细胞,对照组加入等量PSB.采用Transwell小室法检测Genistein作用于TGF-β1诱导Panc-1细胞后侵袭力的变化;RT-PCR技术检测波形蛋白(Vimentin)、E-钙依赖黏附素(E-Cadherin)的mRNA表达;Western blot蛋白印迹法检测E-Cadherin蛋白的表达;应用显微镜观察分析细胞结构的改变.结果:TGF-β1对Panc-1细胞有显著诱导上皮-间质转化的作用,并增加细胞侵袭力,Genistein不但对诱导后Panc-1细胞侵袭力有抑制,对细胞上皮-间质样转化也有抑制,且这种作用呈剂量依赖性.0μmol/L Genistein组细胞计数明显比对照组高(99.16±11.30vs65.46±8.99,P<0.05),50μmol/L Genistein处理诱导后细胞48h,细胞侵袭力下降,Vimentin mRNA表达水平降低,而E-Cadherin mRNA和蛋白表达水平升高,细胞形态学间质样特性被逆转.结论:Genistein具有明显抑制TGF-β1诱导的人胰腺癌细胞Panc-1侵袭力的作用,这可能是其抗侵袭作用的机制之一.AIM： To investigate the effect of Genistein on epithelial-mesenchymal transdifferentiation （EMT） induced by transforming growth factor-β1 （TGF-β1） in human pancreatic cancer cell line Panc-1, and to explore the mechanism of Genistein inhibiting invasion of Panc-1 cells. METHODS： Panc-1 cells were treated with TGF-β1 and Genistein （0, 1, 25, 50 μmol/L）, and those treated with PSB served as controls. Transwell chamber assay was performed to determine the invasion ability change of Panc-1 cells. Reverse transcription-polymerase chain reaction （RT-PCR） was used to estimate the mRNA expression of vimentin and E-cadherin. Western bloting assay was used to measure the protein expression of E-cadherin. Cell structure was observed by microscopy. RESULTS： TGF-β1 obviously promoted EMT and invasion abilibty of Panc-1 cells. Not only the invasion ability but also EMT induced by TGF-β1 were significantly inhibited by Genistein in a dose-dependent manner. The number of Panc-1 cells was larger in 0 μmol/L Genistein group than that in the control group （99.16 ± 11.30 vs 65.46 ± 8.99, P 〈 0.05）. Genistein at concentraion of 50 μmol/L down-regulated the mRNA expression of vimentin and up-regulated the mRNA and protein expression of E-cadherin. The characteristic morphology of EMT was reversed. CONCLUSION： Genistein can inhibit TGF-β1- induced invasion of Panc-1 cells remarkably, which may be one of its anti-invasion mechanisms.

关 键 词：三羟异黄酮 胰腺癌 侵袭 转化生长因子-β1 细胞上皮间质样转化 

分 类 号：R735.9[医药卫生—肿瘤]