黄芩苷对错配修复基因缺失LoVo细胞的抑制作用及其机制  

Inhibitory effect of baicalein on colonic LoVo cell line with deficiency of mismatch repair gene in vitro

作　　者：杨柏霖[1] 刘飞[2] 谷云飞[1] 金黑鹰[2] 刘秀芳[2] 

机构地区：[1]南京中医药大学附属医院肛肠科,江苏省南京市210029 [2]南京中医药大学第三附属医院全国肛肠医疗中心,江苏省南京市210001

出　　处：《世界华人消化杂志》2008年第21期2376-2380,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30572447~~

摘　　要：目的:探讨黄芩苷诱导LoVo细胞凋亡的作用及机制.方法:将黄岑苷40mg溶于19.8mL生理盐水+100μLHCl+100μLNaOH,阿斯匹林1.8g溶于2mL乙腈+4mL NaOH+2m LHCl,分别处理LoVo细胞,1g/L乙腈溶液为对照.采用体外培养技术、TUNEL法观察细胞凋亡率,变性凝胶电泳检测微卫星标志BAT-25、D2S123状况.结果:黄芩苷和阿斯匹林均能诱导LoVo细胞凋亡,在各时间点与对照组比较统计学差异有显著意义(P=0.0000);黄芩对结肠LoVo细胞的诱导凋亡作用明显强于阿司匹林,24h两者差异无统计学意义,96h和1wk两者差异统计学有显著意义(93.33%vs44.23%,63.29%vs23.20%,均P=0.0000),其作用时间在96h达到高峰,与时间不呈依赖关系.黄芩苷和阿司匹林均未影响LoVo细胞的BAT-25、D2S123微卫星状态.结论:黄芩苷具有较强诱导LoVo细胞凋亡的作用,但对LoVo细胞的BAT-25、D2S123微卫星状态无影响,其诱导LoVo细胞凋亡可能是通过其它作用途径.AIM： To explore the influence of baicalein on the apoptosis of colonic LoVo cell line with deficiency of hMSH2 gene as well as its mechnism. METHODS： LoVo cells were treated baicalein （40 mg baicalein＋19.8 mL isotonic＋100μL HCI＋100 μL NaOH）, aspirin （1.8 g aspirin＋2 mL methyl cyanide＋4 mL NaOH＋2 mL HC1） and 1 g/L methyl cyanide （control group）. In vitro cell culture technique and TUNEL method were used to measure the apoptosis rate of LoVo cells. Polymerase chain reaction-single-strand conformarion polymorphism and denaturing gel electrophoresis were employed to detect the status of microsatellite markers BAT-25 and D2S123. RESULTS： Baicalein and aspirin had obvious inhibitory effect on LoVo cells, and the apoptosis rate of LoVo cells was higher than that in the control group at each time point （P = 0.0000）. Baicalein was superior to aspirin in inducing apoptosis of LoVo cells at 96 h and I wk （93.33% vs 44.23%, 63.29% vs 23.20%; both P = 0.0000）. The effect of baicalein reached the peak at 96 h, not in a rime-dependent manner. Microsatellite status in BAT-25 and D2S123 were not affected by baicalein or aspirin. CONCLUSION： Baicalein can induce apoptosis of colonic LoVo cells with deficiency of hMSH2 gene in vitro, without affecting the microsatellite status of BAT-25 and D2S123.

关 键 词：黄芩苷 凋亡 微卫星不稳定 聚合酶链反应-单链构象多态性 

分 类 号：R285.5[医药卫生—中药学]