碘酸钾-淀粉光度法测定水样中生化需氧量(BOD5)  

Determination of biochemical oxygen demand(BOD_5) in water samples by potassium iodate-starch spectrophotometry

作　　者：沙鸥[1] 马卫兴[1] 郭妍 许兴友[1] 彭冬冬[1] 

机构地区：[1]江苏连云港淮海工学院化工系 [2]连云港金兆水务有限公司,江苏连云港222005

出　　处：《冶金分析》2008年第7期23-27,共5页Metallurgical Analysis

基　　金：江苏省高校自然科学研究计划资助项目(05KJB150003);江苏省海洋生物技术重点建设实验室基金资助项目(2005HS010)

摘　　要：生化需氧量(BOD)是环境监测中反映水质好坏的重要参数,本文依据碘酸钾为基准,提出了碘酸钾-淀粉光度测定水样中BOD5的新方法。实验以碘酸钾与过量碘化钾生成单质碘,新生成的单质碘与淀粉生成的蓝色物质在574 nm处有最大吸收,采用光度法测定5天前后培养水样中溶解氧的含量来计算水样中BOD5。所拟方法用于BOD5标准样品溶液、葡萄糖-谷氨酸标准溶液和污水厂进出口水样中BOD5的测定,结果与国家标准方法所得结果相一致。The biochemical oxygen demand（BOD） is an important parameter in reflecting the water quality in the environmental monitoring. The method of determining BOD5 in water by potassium iodate-starch spectrophotometry was built on the reaction between KIO3 which employed as the standard solution and excessive KI. The released I2 could react with the starch to form a blue complex, which had the maximum absorbance at 574 nm. BOD5 in water could be calculated through the amount of dissolved oxygen before and after 5 days determined. The method was used to the determination of biochemical oxygen demand in different water samples, such as BOD5 standard sample, glucose-glutamic acid solution and influent and effluent water from waste water factory and the results were in agreement with those of the national standard method.

关 键 词：生化需氧量 分光光度法 碘酸钾 淀粉 

分 类 号：X832[环境科学与工程—环境工程]