沙立度胺对乳腺癌细胞血管内皮生长因子表达的影响  

Effect of thalidomide on the expression of VEGF mRNA in human breast cancer cell lines

作　　者：李书芹[1] 季艳霞[1] 杨志勇[1] 李海燕[1] 张振军[1] 贾军刚[1] 万良刚[1] 

机构地区：[1]邯郸市中心医院放疗科,河北省邯郸056001

出　　处：《中国基层医药》2008年第7期1057-1058,1233,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：河北省科技攻关项目（062761401）

摘　　要：目的观察沙立度胺对人乳腺癌细胞系MCF-7、MDA-MB-231细胞血管内皮生长因子（VEGF）表达的抑制作用。方法应用半定量逆转录-聚合酶链反应（RT—PCR）技术，分别观察沙立度胺对MCF-7和MDA-MB-231细胞VEGF表达的抑制作用。结果沙立度胺在50μg／L明显抑制两株细胞VEGF mRNA的表达，但浓度增高到100μg／L抑制VEGF mRNA表达的作用反而减弱。结论沙立度胺在一定浓度范围内能抑制乳腺癌细胞VEGF mRNA的表达。Objective To assess the effects of thalidomide on VEGF mRNA level in human breast cancer cell lines. Methods Breast cancer cells were treated with thalidomide for 24-72h, RT-PCR was uced to detect the level of VEGF mRNA expression. Results When the concentration of drug is 50μg/L,thalidomide strongly inhibited the level of VEGF mRNA expression. Conclusion Within certain concentration range of thalidomide can inhibit the level of VEGF mRNA expression in breast cancer cell.

关 键 词：乳腺肿瘤 沙立度胺 血管内皮生长因子 细胞系 

分 类 号：R737[医药卫生—肿瘤]