妊娠高血压综合征患者血小板／T红细胞活化抗原1的表达及意义  

The expression, and significance of PTA1 in patients with pregnancy-induced hypertension syndrome

作　　者：许瑞环[1] 廖长征[1] 尹学念[1] 陈思祖[1] 徐文莉[1] 李康[1] 

机构地区：[1]深圳市龙岗中心医院中心实验室,广东省深圳518116

出　　处：《中国基层医药》2008年第7期1067-1069,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：广东省深圳市龙岗区科技基金资助项目（200229）

摘　　要：目的研究血小板／T红细胞活化抗原1（PTA1）的表达与妊娠高血压综合征发生、发展的关系。方法用流式细胞术观察妊娠高血压综合征患者血清对于体外培养血管内皮细胞表达PTA1的作用以及该血管内皮细胞和活化血小板粘附后PTA1表达的变化。结果妊娠高血压综合征患者血清刺激血管内皮细胞表达PTA1为15．51％（24h）、6．32％（48h），对照组为7．81％（24h）、4．72％（48h），差异有统计学意义（P〈0．01）；活化血管内皮细胞和活化血小板孵育后，血管内皮细胞表达PTA1为6．64％（24h）、4．13％（48h）。妊娠高血压综合征患者血清刺激血管内皮细胞表达PTA1在用PTA1／IgG阻断后为8．11％（24h）和4．28％（48h），与阻断前比较明显降低（P〈0．01）。结论妊娠高血压综合征患者体内存在某种非正常刺激因素，使血管内皮细胞敏感性增强，更易于活化。妊娠高血压综合征患者血清活化的血管内皮细胞与活化血小板孵育后表达的PTA1明显降低，提示PTA1分子直接或间接参与了妊娠高血压综合征血管内皮细胞和血小板的粘附。Objective To explore the relationship of the expression, regulation and adhesion of PTA1 in pregnancy induced hypertension（PIH）. Methods The expression,regulation and adhesion of PTA1 on vascular endothelial cells incubated by sera from 10 PIH patients and 10 normal pregnant women repectively was detected by flow cytometry. Results The positive rate of PTA1 15.51% （24h） and 6.32 % （48h） in PIH group is significantly higher than that 7.81% （24h） and 4.72 % （48h） in normal pregnant woman group（ P 〈 0.01 ）. After the incubation with platelets,the positive rate of PTA1 6.64 % （24h） and 4.13 % （48h） in PIH group is lower than before（ P 〈 0.05）. The PTA1 expression 8.11% （24h） and 4.28% （48h） in PIH group is much lower after blocking with PTA1/IgG than that before（ P 〈 0.01）. Conclusion There are some abnormal stimulated factors in PIH patients, which activate vascular endothelial cells. PTA1 plays an important role in the adhesion of platelets to vascular endothelial cells, PTA1 is closely related to the progress of PIH which indicates that PTA1 directly or indirectly takes part in the pathophysiologic consequences of PIH.

关 键 词：妊娠中毒症 EPH 血小板活化因子 E选择素 流式细胞术 

分 类 号：R714[医药卫生—妇产科学]