高效液相色谱法同时测定化妆品中氢醌、苯酚、抗生素和防腐剂等11种物质  

Determination of hydroquinone,phenol,antibiotics and preservatives in cosmetics by HPLC

作　　者：张玉黔[1] 李宏志 贾薇[1] 权伍英[1] 栾燕[1] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031 [2]辽宁省药品检验所,沈阳110023

出　　处：《中国卫生检验杂志》2008年第8期1548-1550,共3页Chinese Journal of Health Laboratory Technology

基　　金：沈阳市科学技术计划项目

摘　　要：目的：建立高效液相色谱（HPLC）法测定化妆品中苯酚、氢醌、抗生素和防腐剂的检测方法。方法：采用高效液相色谱（HPLC）法,以甲醇-0.01 mol/L磷酸二氢钠溶液（pH=4.35）为流动相,采用梯度洗脱方式,同时测定化妆品中苯酚、氢醌、抗生素和防腐剂。结果：各物质在1～200μg/ml范围内呈良好线性关系（r〉0.998）,平均回收率85.2%～114.4%,精密度良好,RSD〈6%。结论：方法灵敏度高,准确度好,操作简单,实用性强,可用于化妆品中苯酚、氢醌、抗生素和防腐剂的检测。Objective： To develop a HPLC method for determination of hydroquinone,phenol,antibiotics and preservatives in cosmetics.Methods：By using the HPLC method,mobile phase was methanol-0.01 mol/L NaH2PO4（pH=4.35）and gradient elution was used to determine hydroquinone,phenol,antibiotics and preservatives in cosmetics.Results：They showed good linearity within the range of 1～200 μg/ml（r〉0.998）,average recovery was 85.2%～114.4%,RSD〈6%.Conclusion：The HPLC method is simple,sensitive,and accurate and could be applied for determination of hydroquinone,phenol,antibiotics and preservatives in cosmetic preparations.

关 键 词：高效液相色谱法 氢醌 苯酚 抗生素 防腐剂 化妆品 

分 类 号：O657.63[理学—分析化学]