纳豆芽孢杆菌对瘤胃微生物发酵的影响  

Effect of Supplement Bacillus subtilis Natto on Ruminal Microbial Fermentation in vitro

作　　者：邓露芳[1] 王加启[1] 姜艳美[1] 刘亮[1] 卜登攀[1] 周凌云[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《畜牧兽医学报》2008年第8期1062-1068,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国际科技合作重点项目(2004DFA06700)

摘　　要：本试验通过人工瘤胃持续发酵体系,研究添加不同浓度纳豆芽孢杆菌对瘤胃微生物发酵的影响。本研究设3个处理组,空白对照组(CK),添加人工瘤胃发酵罐容积1%的纳豆芽孢杆菌处理1组(TR1)和添加人工瘤胃发酵罐容积5%的纳豆芽孢杆菌处理2组(TR2)。试验结果表明,添加纳豆芽孢杆菌处理组有提高瘤胃pH的趋势,但差异不显著(P>0.05)。TR2组NH3-N浓度始终高于对照组(CK)(P<0.05),但TR1与CK比较没有显著差异(P>0.05)。本试验中TR1和TR2组微生物蛋白(MCP)值均显著低于CK组(P<0.05)。与CK组相比较,添加纳豆芽孢杆菌处理组挥发性脂肪酸(VFA)各指标均有不同程度的提高,其中TR2组乙酸、丙酸、丁酸和挥发性脂肪酸总量分别提高6.52%、1.96%、11.02%和5.77%(P<0.05),乙酸∶丙酸比例显著上升(P<0.05);但TR1和TR2组之间,TR2组除了丁酸含量显著高于TR1组(P<0.05)外,其余指标差异均不显著。研究结果表明,纳豆芽孢杆菌对瘤胃氮的转化利用有一定负面影响,但提高了瘤胃碳水化合物的消化代谢。The purpose of the present study was to examine the effect of different concentration of Bacillus subtilis natto on ruminal microbial fermentation in persistent artificial rumen system. Three groups, including control group （CK）, treatment 1 group （TR1） supplied 1% B. subtilis natto of fermenter capacity and treatment 2 group （TR2） supplied 5% B. subtilis natto of fermenter capacity were employed. Results indicated that, compared with CK, pH values of TR1 and TR2 increased, but the difference was not significant （P〉0.05）. The NH3-N concentration of TR2 was always higher than that of CK （P〈0.05）, while TR1 failed to increase NH3-N concentration significantly （P〉0.05）. Compared with CK, the MCP concentration of TR1 and TR2 decreased significantly （P〈0.05） and their concentration of acetate, propionate, butyrate, and total volatile fatty acids all increased to different extent. Compared with CK, the concentration of acetate, propionate, butyrate, and total volatile fatty acids in TR2 increased by 6.52%, 1.96%, 11.02% and 5.77% （P〈0.05）, respectively. TR2＇s acetate/propionate ratio also increased significantly （P〈0.05）. However, no significant difference was observed between TR1 and TR2（P〉0.05） in all volatile fatty acids except that b that inTR1 （P〈0.05）. The results suggested th metabolism, but could enhance the carbohydrate d utyrate concentration was higher in TR2 than at B. subtilis natto has a negative effect on N igestion by rumen microbes.

关 键 词：纳豆芽孢杆菌 人工瘤胃发酵 瘤胃微生物 

分 类 号：S827.5[农业科学—畜牧学]