恶性滋养细胞肿瘤p16抑癌基因的表达与细胞增殖的关系及临床意义  

Relationship between p16 Protein Expression and Cell Proliferation in Malignant Trophoblastic Tumors and Clinical Significance

作　　者：符淳[1] 张琼英[1] 卢放根[1] 张志胜[1] 林礼茂[1] 

机构地区：[1]湖南医科大学附属第二医院

出　　处：《湖南医学》1997年第6期325-326,共2页Hunan Medical Journal

摘　　要：为研究ｐ１６抑癌基因表达与滋养细胞癌变的关系，用免疫组化ＳＰ法测定了ｐ１６、增殖细胞核抗原（ＰＣＮＡ）在恶性滋养细胞肿瘤、葡萄胎及正常绒毛中的表达。结果示：①ｐ１６蛋白表达在恶性滋养细胞肿瘤和正常绒毛间差异有显著性（Ｐ＜００５）；②ｐ１６阴性标本较ｐ１６阳性标本的ＰＣＮＡ阳性率显著高（Ｐ＜００５）；③不同临床分期的恶性滋养细胞肿瘤患者ｐ１６阳性率不同，其中ｐ１６阳性患者３年生存率高于ｐ１６阴性者。以上结果提示，ｐ１６基因的突变可能是滋养细胞癌变及增殖失控的重要原因；临床检测ｐ１６蛋白表达对估计恶性滋养细胞肿瘤患者的预后有帮助。o explore the correlation between p16 protein expression and the carcinogenesis of trophoblastic cell,the expression of p16 protein in malignant trophoblastic tumors,hydatidiform mole and normal villi was detected using immunohistochemical methods. It showed①In contrast to normal villi,the expression of p16 protein in malignant trophoblastic tumors was significantly different(P<0.05);②The positive rate of the proliferation cell nuclear antigen (PCNA) in p16 negative samples was markedly higher than that in p16 positive samples(P<0.05);③The different clinical phases of the malignant trophoblastic tumors were dissimilar in the positive rate of p16 protein. Patients with positive p16 expression had a higher three-year survival rate than those with negative expression.All these facts indicate that p16 gene mutation may be an important factor in carcinogenesis of trophoblastic cells and outcontroled proliferation and the detection of p16 protein expression may be helpful to evaluate the prognosis of malignant trophoblastic tumors.

关 键 词：滋养细胞肿瘤 恶性 P16 抑癌基因 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]