多重聚合酶链反应快速检测嗜水气单胞菌和爱德华菌  

Rapid defection Aeromonas hydrophila and Edwardsiella by multiplex polymerase chain reaction

作　　者：邓显文[1] 谢芝勋[1] 谢志勤[1] 刘加波[1] 庞耀珊[1] 谢丽基[1] 

机构地区：[1]广西兽医研究所,南宁530001

出　　处：《中国人兽共患病学报》2008年第8期752-754,共3页Chinese Journal of Zoonoses

基　　金：广西科技攻关项目(桂科攻0428001-2);国家百千万人才工程专项资金(945200603)联合资助

摘　　要：目的根据嗜水气单胞菌株和爱德华菌株16S rDNA基因的结构特点,设计合成了二对引物XZAH3、XZAH4和XZE7b、XZE8,建立了一种同时检测鉴别嗜水气单胞菌株和爱德华菌株的多重PCR技术。试验结果表明,用这两对引物对嗜水气单胞菌和爱德华菌株进行多重PCR,嗜水气单胞菌株只扩增出361bp一条带,而爱德华菌株只扩增出576bp一条带,而对其他鱼病病原的扩增不出现任何条带,结果均为阴性;敏感性测定结果表明,该多重PCR最低能检出10pg的嗜水气单胞菌株、爱德华菌株的DNA模板。The multiplex polymerase chain reaction （PCR） was developed and optimized to detect and identify Aeromonas hydrophila and Edwardsiella simultaneously. Two pairs of specific primers XZAH3 ,XZAH4 and XZE7b. XZE8 were designed according to the sequences of 16S rDNA of Aerornonas hydrophila and Edwardsiella. The results showed that only 361pb-long DNA fragment for the Edwardsiella strains was amplified and the 576pb-long DNA fragments for the Aerornonas hydrophila were amplified by the multiplex PCR with these two pairs of primers, but the other fish pathogenic viruses and bacteria were not amolified. As little as 10 pg DNA of Aerornonas hvdrophila and Edwardsiella could be detected by the multiolex PCR.

关 键 词：多重PCR 检测 嗜水气单胞菌 爱德华菌 

分 类 号：R155.31[医药卫生—营养与食品卫生学] R446.5[医药卫生—公共卫生与预防医学]