人B7-1分子对小鼠淋巴细胞活化的辅助刺激作用  

Costimulatory Effect of Human B71 Molecule on Activation of Mouse Lymphocyte Cells

作　　者：杨金亮[1] 黄晋生[1] 严明山[1] 王顺友 张海荣[1] 连慕兰[1] 乔媛媛[1] 郭启煜[1] 房殿春 

机构地区：[1]海军总医院中心实验科

出　　处：《基础医学与临床》1997年第6期55-58,共4页Basic and Clinical Medicine

基　　金：国家教委细胞增殖与调控生物学开放实验室开放课题

摘　　要：本文用脂质体法将人Ｂ７－１基因导入小鼠前胃癌细系ＦＣ及人白血病细胞系ＨＬ６０，获得了稳定高效表达Ｂ７－１分子的ＭＦＣ和ＨＬ６０克隆（分别命名为Ｂ７和ＨＬ６０－Ｂ７）。在ＭＦＣ－Ｂ７及ＨＬ６０－Ｂ７刺激鼠脾淋巴细胞的增殖实验中，野生型肿瘤细胞中只能引起微弱的增殖反应，而转染Ｂ７－１分子的肿瘤细胞能非常显著刺激脾细胞的增殖（Ｐ＜０．００１）。这种效应能被抗Ｂ７－１单抗所阻断。结果表明人Ｂ７－１分子能为鼠淋巴细胞的活化增殖提供辅助刺激信号。e transfected human B71(CD80) gene to mouse gastric carcinoma cell line MFC and human leukemia cell line HL60 with lipofectamin. The tumor cell clones of high expression B71 molecule were screened by immunohistochemstry method (named MFCB7 and HL60B7 respectively).Compared with MFC and HL60 cells ,MFCB7 and HL60B7 could significantly stimulate proliferation of mouse spleen cells respectively(P<0.001).The stimulate effects could be inhibited by antiB71[KG*4〗mAb(BB1). The results suggest that human B71 molecule can costimulate proliferation of mouse spleen cells.

关 键 词：B7-1分子 淋巴细胞 增殖效应 肿瘤细胞 

分 类 号：R730.3[医药卫生—肿瘤]