阻断缝隙连接通讯对局灶性脑缺血后海马迟发性神经元死亡及脑梗死体积的影响  

Effect of Blocking Gap Junction Communication on Delayed Neuronal Death in Rat Hippocampus and Infarcted Volume After Focal Cerebral Ischemia

作　　者：唐颖馨[1] 徐沙贝[1] 易陈菊[1] 张强[1] 骆翔[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2008年第4期187-189,209,共4页Journal of Critical Care In Internal Medicine

基　　金：国家自然科学基金资助项目(30600187);国家杰出青年科学基金资助项目(30725019);湖北省自然科学基金资助项目(2007ABA174)

摘　　要：目的:探讨阻断缝隙连接通讯对大鼠局灶性脑缺血后海马迟发性神经元死亡及脑梗死体积的影响。方法:大鼠左侧脑室注射缝隙连接阻断剂甘珀酸,对照组左侧脑室注射生理盐水,2h后颈内动脉插线法制备大脑中动脉缺血再灌注模型,采用DNA原位末端标记TUNEL技术及红四氮唑(TTC)染色,观察阻断缝隙连接通讯对大鼠局灶性脑缺血3d后海马迟发性神经元死亡及脑梗死体积的影响。结果:不给予缝隙连接阻断剂,大脑中动脉缺血模型大鼠有45%在术后3d出现海马迟发性神经元死亡;用甘珀酸阻断缝隙连接后30%的大鼠出现海马迟发性神经元死亡,几率明显减小(P<0.01);与对照组相比,干预组的脑梗死体积明显减少(P<0.01)。结论:阻断缝隙连接通讯,局灶性脑缺血后海马迟发性神经元死亡的发生率降低,脑梗死体积减小。Objective：To explore the effect of blocking gap junction communication after focal cerebral ischemia on delayed neuronal death （DND） in the ipsilateral hippocampus and the infarcted volume. Methods： A rat model of middle cerebral artery occlusion was made by the intraluminal occlusion technique, Carbenoxolone, a gap junction blocker, was used to block gap junction communication, and the effect of blocking gap junction communication after focal cerebral ischemia on DND in the ipsilateral hippocampus and the infarcted volume was observed by TUNEL labeling and TTC staining methods, Results： Without inhibition of gap junction communication, DND was detected in 45% of the animals in hippocampal CA1 region 3 days after middle cerebral artery occlusion. With the inhibition of gap junction communication, DND appeared in only 30% of the animals. The infarcted volume was significantly smaller in the experimental group than that of control group （P〈0.01）. Conclusion： Blocking gap junction communication can reduce the incidence of DND in hippocampus and the infarcted volume after middle cerebral artery occlusion.

关 键 词：缝隙连接 局灶性脑缺血 迟发性神经元死亡 脑梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]