Peat1蛋白及其3个缺失突变体的表达与热稳定性分析  

Expression and Thermal Stability Analysis of Peat1 and the 3 Deletion Mutants

作　　者：李明勇[1] 邱德文[1] 曾洪梅[1] 杨秀芬[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100081

出　　处：《安徽农业科学》2008年第20期8482-8483,8534,共3页Journal of Anhui Agricultural Sciences

基　　金："973"项目(2003CB114204);北京市科技计划项目(D0706005040-431)

摘　　要：[目的]为揭示Peat1蛋白序列(含1个NAC结构域和1个UBA结构域)各片段在该蛋白热稳定性上的作用。[方法]构建了Peat1蛋白及其3个缺失突变体的融合表达载体,经IPTG诱导表达的目标蛋白,对所得蛋白经AKTA亲和纯化和热稳定性分析。[结果]与Peat1一样,3个缺失突变体蛋白(Peat1、Peat1-△CD99、Peat1-△ND49和Peat1-△ND108)都具有良好的热稳定性。[结论]说明NAC或UBA结构域对Peat1蛋白的热稳定性不是同时必需的,可能它们都有单独赋予蛋白某种特殊稳定结构的作用。[Objective] The research aimed to reveal the functions of NAC and UBA domains in Peat1＇s thermal stability.[Method] Fusion expression vectors of Peat1 protein and the 3 deletion mutants were constructed.The recombinant plasmids were induced by IPTG and the target proteins（Peat1,Peat1-△CD99,Peat1-△ND49 and Peat1-△ND108）were expressed obtained by AKTA and its thermal stability was analyzed.[Result] The research found that 3 deletion mutants have good thermal stability like Peat1.[Conclusion] The research demonstrated that the coexistence of NAC or UBA domains is not necessary to thermal stability of Peat1 protein,and they may give the protein particular stability structure seperately.

关 键 词：Peat1 缺失突变 热稳定性 

分 类 号：Q819[生物学—生物工程]