牦牛IL-4基因的克隆及其遗传演化关系分析  

Cloning and Sequence Analysis of Yak Interleukin-4 gene

作　　者：何延华[1] 房永祥[2] 陈国华[2] 段风云[2] 王生富[1] 李小庆[2] 景志忠[1] 

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省寄生虫病重点实验室,甘肃兰州730046

出　　处：《动物医学进展》2008年第8期24-28,共5页Progress In Veterinary Medicine

基　　金：国家高新技术"863"项目(2006AA10A203);甘肃省自然基金项目(3ZS061-A25-081)

摘　　要：克隆牦牛白细胞介素-4（IL～4）基因，并对其进行遗传演化分析。从刀豆素（ConA）和脂多糖（LPS）联合刺激培养的牦牛外周血淋巴细胞提取总RNA．利用RT—PCR方法扩增牦牛IL-4全长cDNA，将其克隆到pMD18～T载体上，测序后进行序列分析。成功克隆到牦牛IL-4基因全序列，序列分析表明，克隆的牦牛IL-4基因序列与GenBank所登录的牛IL-4核苷酸序列及推导的氨基酸序列的同源性分别这99．8％和100％，与人、猕猴、猪、山羊、马等物种的核苷酸及其推导氨基酸序列的同源性分别在44．4％～96．3％和27．4％～91．1％之间。在国内首次成功地从牦牛外周血淋巴细胞中克隆到IL-4的基因，其ORF为408bp，推导编码135个氨基酸。Cloning and genetic analysis of yak interleukin-4 gene were conducted. The full length cDNA of boiL-4 was amplified by RT-PCR from the yak peripheral blood lymphocyte stimulated by ConA and LPS, and was cloned in pMD18-T vector and sequenced. Homologies of nucleotide and deduced amino acid sequences between the yak IL-4 and registered boiL4 in the GenBank are 99.8% and 100%,respectively. Homologies of yak IL-4 nucleotide sequence compared with Homo, Macaca, Sus scrofa, Capra and Equus are in 44.4%-99.8%. Homologies of amino acid sequence of them are in 27.4%-91.1%. In conclusion, IL- 4 containing an ORF of 408 bp and coding 135 amino acids was first cloned successfully from the yak PBMC in China.

关 键 词：白介素4 克隆 序列分析 牦牛 

分 类 号：S858.23[农业科学—临床兽医学] S814.8[农业科学—兽医学]