新基因MIP2的克隆和特性分析  

Cloning and characterization of a novel gene

作　　者：蒋磊[1] 王慷慨[1] 袁灿[1] 陈广文[1] 刘可[1] 王桂良[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,湖南长沙410078

出　　处：《中国现代医学杂志》2008年第15期2142-2145,共4页China Journal of Modern Medicine

基　　金：国家重点基础研究发展规划项目(973)(No:G2000056908);国家自然科学基金项目(No:30170373;No:30300345)

摘　　要：目的克隆1个大鼠心肌缺血-再灌诱导表达上调的新基因MIP2,并分析其相关特性。方法采用生物信息学方法,克隆了1个大鼠心肌缺血-再灌诱导表达上调的新基因MIP2,分析了新基因的若干特性;采用PCR从cDNA文库中克隆MIP2的开放阅读框。结果MIP2定位于大鼠染色体1q42.12,含14个外显子和13个内含子,开放阅读框(ORF)为1497bp,编码498个氨基酸。在其编码蛋白的N-端含1个CTLH结构域,C-端有5个WD重复序列;MIP2的开放阅读框经测序证实与预测的完全一样;多组织膜RNA印迹证实了MIP2转录本的存在,且显示该基因在心与骨骼肌表达最高,其次是脾和睾丸,在其他组织表达较低或无表达。结论成功克隆了1个大鼠心肌缺血-再灌诱导表达上调的新基因MIP2。[Objective] To clone a new gene MIP2 which is up-regulated during rat myocardial isehemia-reperfusion. [Methods] Bioinformaties was used to clone and characterize a novel gene. Multi-tissue membrane was used to analyze the expression of the gene. PCR was used to clone the open reading frame of the gene. [Results] The open read frame of MIP2 was proved by PCR and DNA sequencing. Bioinformaties analysis indicated that MIP2 is located at chromosome lq42, including 14 exons and 13 introns. The full-length eDNA has an open reading frame of 1497 bp , which encodes 498 amino acids. There are a CTLH domain at the N terminal of the hypothetical protein and 5 WD40 repeats at the C terminal of the protein. MIP2 is expressed abundantly in skeleton muscle and heart, seldom in other tissues. [Conclusion] A novel gene up-regulated during rat myocardial ischemia-reperfusion has been successfully cloned and it is worthwhile to further study the biological functions of MIP2.

关 键 词：新基因 MIP2 生物信息学 克隆 

分 类 号：R-332[医药卫生] R318.04