选择性环氧合酶-2抑制剂对实验性胃溃疡大鼠胃黏膜增殖状态的影响  

Effect of selective COX-2 inhibitor on gastric epithelial cell proliferation in rats with experimental ulcer

作　　者：何美蓉[1] 林劲秋[2] 宋于刚[1] 赖卓胜[1] 

机构地区：[1]南方医科大学南方医院消化内科,广东广州510515 [2]南方医科大学护理学院,广东广州510515

出　　处：《中国现代医学杂志》2008年第15期2163-2165,2169,共4页China Journal of Modern Medicine

基　　金：广东省自然科学基金(No:04020400)

摘　　要：目的明确选择性环氧合酶-2(COX-2)抑制剂对实验性大鼠胃溃疡愈合的影响,并从胃黏膜增殖状态的角度探讨其延缓胃溃疡愈合的机制。方法以乙酸性大鼠胃溃疡模型为基础,观察选择性COX-2抑制剂塞来昔布对胃溃疡愈合的影响及其对胃黏膜增殖细胞核抗原(PCNA)、肝细胞生长因子(HGF)及其受体c-Met蛋白表达的影响。结果制模术后第9日,生理盐水组和塞来昔布组的溃疡面积分别为(11.9±3.1)mm2和(19.7±3.8)mm2(P<0.01);制模术后第6日和第9日,塞来昔布组PCNA标记指数(PCNALI)和c-Met蛋白水平显著低于生理盐水组,而HGF蛋白水平差异则无显著性。结论选择性COX-2抑制剂能显著延缓实验性大鼠胃溃疡的愈合,其机理之一可能是通过下调c-Met蛋白表达抑制胃黏膜上皮细胞的增殖。[Objective] To determine whether selective COX-2 inhibitor affects the healing of experimental rats gastric ulcer and elucidate the mechanisms from the angle of gastric epithelial cell proliferation. [Methods] Acetic acid-induced ulcers were caused by application of acetic acid to the serosal surface of the anterior face of the rats gastric bodies, then the effects of the selective COX-2 inhibitor, Celecoxib, on the healing of gastric ulcer, and on the protein expression of PCNA, HGF, c-Met were observed. [Results] Nine days after ulcer induction, the ulcer area was respectively （11.9±3.1） mm^2 and （19.7±3.8） mm^2 in NS group and Celecoxib group（P 〈0.01）. The protein expression of PCNA and HGF in Celecoxib group were significantly lower than that in NS group at both 6 and 9 days after ulcer induction, while there weren＇t distinct difference between the two groups in the protein expression of HGF. [Conclusion] Selective COX-2 inhibitor can significantly delay the healing of experimental rats gastric ulcer, and one of the mechanisms may be inhibiting gastric epithelial cell proliferation through downregulation of the pro- tein expression of c-Met.

关 键 词：环氧合酶 胃溃疡 增殖细胞核抗原 肝细胞生长因子 

分 类 号：R573.1[医药卫生—消化系统]