不同控制模式对毕赤酵母表达IL-2-HSA的比较研究  

Comparison of different control modes in Pichia pastoris expressing interleukin-2-human serum albumin(IL-2-HSA)

作　　者：堵益平[1] 高敏杰[1] 金虎[1] 史仲平[1] 

机构地区：[1]江南大学生物工程学院工业微生物教育部重点实验室,无锡214122

出　　处：《生物学杂志》2008年第4期44-47,61,共5页Journal of Biology

摘　　要：以溶氧（DO）和甲醇浓度作为控制指标，分别考察了诱导表达阶段DO和甲醇浓度对毕赤酵母高密度发酵表达白介素-2-白蛋白融合蛋白（IL-2-HSA）的影响。通过实验比较得出：诱导阶段控制DO对IL-2-HSA表达影响不明显，而甲醇浓度对IL-2-HSA影响很大。高甲醇浓度（15～25g／L）有利于IL-2-HSA的表达，发酵结束时的表达量约是低甲醇浓度（5～8g／L）诱导下的2倍。此外，在诱导阶段，通过限制性添加甘油可以有效提高菌体的呼吸活性，促进菌体生长。The effects of DO and the methanol concentration on interleukin-2-human serum albumin（IL-2-HSA） expression by Pichia pastoris during the induction were investigated. The research suggested that DO had little effect for IL-2-HSA expression, and the methanol concentration played an important role in IL-2-HSA expression process. A high methanol concentration （15-25 g/L） control was more beneficial to IL-2-HSA expression,which was about as two times as the expression level compared with that expressed under low methanol concentration （5-8 g/L） control at the end of fermentation. In addition,limited co-feeding of glycerol in induction stage could effectively improve cell respiratory activity and promote cell growth.

关 键 词：白介素-2-白蛋白融合蛋白 毕赤酵母 溶氧 甲醇浓度 

分 类 号：Q819[生物学—生物工程]