UpG法用于辐射损伤的量效关系研究  

Study on relationship of dose-response using the ultraviolet plus Giemsa technology

作　　者：彭晓梅[1] 曹建平[1] 朱巍[1] 樊赛军[1] 王小强[1] 黄晓菲[1] 刘杨[1] 陈遐林[1] 宫晓梅[1] 曾静[1] 

机构地区：[1]苏州大学放射医学与公共卫生学院,苏州215123

出　　处：《辐射研究与辐射工艺学报》2008年第4期233-236,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(30670632)资助

摘　　要：应用常规染色体制作法和紫外线照射加吉姆萨染色法(Ultraviolet plus Giemsa technology,UpG)检测经60Coγ射线离体照射后的人外周血淋巴细胞染色体畸变率(双着丝粒体+环),研究其剂量-效应关系,探讨UpG法用于辐射损伤的剂量估算效果。实验结果表明:两种方法得出的染色体畸变率与照射剂量密切相关(P<0.05),均可用于辐射损伤的量效关系研究。两种方法的结果相比,在同一照射剂量下,UpG法高于常规法,具有显著性差异(P<0.05)。UpG法可以很好用于辐射损伤的剂量估算中,且较为准确、灵敏。To detect the rate of lymphocyte Chromosome aberration by conventional chromosome production and ultraviolet plus Giemsa technology （UpG）, after the human peripheral blood irradiated by ^60Co γ-ray, and the dose-response relationship of the rate of chromosome aberration was discussed. The feasibility of UpG applied in dose estimation was discussed. The results show that the rate of chromosome aberration is closely related to dose （P〈0.05）.Compared the conventional method to UpG, the result of UpG is higher, and a extremely statistically difference was observed between two methods （P 〈 0.05）. These results show that a well relationship of dose-response can be established by UpG, and accurate and sensitive.

关 键 词：染色体畸变 UpG法 剂量效应 

分 类 号：R811.5[医药卫生—放射医学]