DNA共价修饰单壁碳纳米管电极的制备及与VB_6相互作用的研究  

Studies on the Preparation of DNA-SWNTs Modified Electrode and Its Interaction with VB_6

作　　者：张怀[1] 张云怀[1] 李静[1] 肖鹏[2] 乔雷[1] 李泽全[1] 樊小燕[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学数理学院,重庆400030

出　　处：《分析测试学报》2008年第8期830-834,共5页Journal of Instrumental Analysis

基　　金：重庆市自然科学基金资助项目(CSTS2005BB4200);重庆大学研究生科技创新基金资助项目(200701Y1A0140200)

摘　　要：利用单壁碳纳米管(SWNTs)上的羧基与DNA末端的氨基形成酰胺共价键从而共价修饰SWNTs,制备了新型DNA共价修饰SWNTs电极。傅立叶红外光谱表明DNA共价结合在SWNTs上,用扫描电镜(SEM)表征了SWNTs、DNA共价修饰的SWNTs的结构与形貌。循环伏安法(CV)研究了DNA修饰SWNTs电极的电化学特性,通过改变扫描速率,发现DNA修饰电极的过程属于扩散控制过程。维生素B6(VB6)与SWNTs上DNA的相互作用研究结果表明:DNA分子共价修饰在SWNTs上后仍具有生物活性,且能够与其他生物分子发生相互作用。A novel DNA- SWNTs （single-wall carbon nanotubes）modified electrode was prepared in this paper. The DNA was linked by forming covalent amide bonds between carboxyl groups of the nanotubes and amino groups at the ends of the DNA oligonucleotides. IR spectra and SEM micrographs for DNA - modified SWNTs revealed that DNA was covalently linked to the surface of SWNTs. Electrochemical characteristics of DNA - SWNTs modified electrode have been investigated by cyclic vohammetry. It was suggested that the electrochemical process was controlled by diffusion. The interaction between DNA - SWNTs modified and VB6 was also studied. The experimental results imply that the DNA molecules modified on the surface of SWNTs can retain its bioactivity showing the ability to interact with other bio-molecules.

关 键 词：单壁碳纳米管 脱氧核糖核酸 修饰 维生素B6 电化学 

分 类 号：O657.15[理学—分析化学] Q563.3[理学—化学]