实时荧光定量PCR技术检测食管鳞癌c-myc mRNA的表达  

Detection of c-myc mRNA in esophageal squamous cell carcinoma by Real-time FQ-PCR

作　　者：孙秀静[1] 朱圣韬[2] 王文海[2] 贺凌婕[2] 徐有青[1] 张澍田[2] 

机构地区：[1]首都医科大学附属北京天坛医院,北京100050 [2]首都医科大学附属北京友谊医院

出　　处：《山东医药》2008年第19期16-18,共3页Shandong Medical Journal

基　　金：北京市科技计划课题任务(Y0905001040331)

摘　　要：目的探讨c-myc mRNA表达与食管鳞癌发病的关系。方法采用实时荧光定量PCR法检测24例食管鳞癌患者食管鳞癌组织和正常食管鳞状上皮组织、癌旁食管鳞状上皮组织中c-myc mRNA的表达。结果食管鳞癌组织与癌旁食管鳞状上皮组织c-myc基因的表达量相近,二者均明显高于正常食管鳞状上皮组织(P<0.05)。结论c-myc基因在食管鳞状上皮癌变过程的早期阶段起重要作用。Objective To investigate the relationship between the expression of c-myc mRNA and human esophageal squamous cell carcinoma （ESCC）. Methods Total RNA was extracted with Trizol from the tissue and mRNA was tran- scribed reversely into eDNA. Real-time fluorescence quantitative reverse transeriptase-polymerase chain reaction （FQ- PCR） was applied to detect e-mye mRNA expression in ESCC, paraeancemus esophageal squamous epithelium and normal squamous epithelium. Results e-mye mRNA in ESCC and paracaneemus esophageal squamous epithelium were expressed approximately, and both were higher than that in adjacent normal esophageal squamous epithelium. Conclusion c-myc gene may play an important role in the carcinogenesis of human ESCC.

关 键 词：基因 C-MYC 食管肿瘤 实时荧光定量PCR 

分 类 号：R735.1[医药卫生—肿瘤]