卵巢浆液性癌B7-H4的表达及临床病理意义  

Expression of B7-H4 in ovarian serous carcinoma and the association with clinicopathological variables.

作　　者：魏双燕[1] 姜洁[1] 南芳芳[2] 成磊[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012 [2]滨州医学院附属医院妇产科

出　　处：《现代妇产科进展》2008年第7期493-496,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:30672233);山东省自然科学基金资助项目(No:Y2006C64)

摘　　要：目的：探讨B7-H4在卵巢上皮性肿瘤浆液性癌中的表达及其临床病理意义。方法：用逆转录聚合酶链反应（RT—PCR）技术及免疫组织化学染色的方法检测6例良性卵巢上皮性肿瘤、10例交界性卵巢上皮性肿瘤、41例卵巢浆液性癌、5例内膜样癌、2例透明细胞癌和10例粘液性癌标本中B7-H4mRNA及蛋白表达，并结合临床病理资料对其中41例卵巢浆液性癌中B7-H4蛋白的表达进行分析。结果：B7-H4mRNA在74例卵巢上皮性肿瘤中均有表达，B7-H4蛋白在良性卵巢上皮性肿瘤、交界性卵巢上皮性肿瘤、恶性卵巢上皮性肿瘤中的表达阳性比例分别为2／6、6／10、52／58，恶性标本阳性率明显高于非恶性标本，差异有统计学意义（P〈0．05）；58例恶性卵巢上皮性癌标本中浆液性癌、内膜样癌、透明细胞癌、黏液性癌的B7-H4蛋白阳性比例分别为41／41、5／5、2／2、4／10，前三种病理类型B7-H4蛋白阳性率明显高于最后一种，且差异有统计学意义（P〈0．05）；41例卵巢浆液性癌标本中B7-H4蛋白阳性细胞比例在〈10％，〉10％～50％，〉50～80％，〉80～100％4组中的分布差异无显著性（P〉0．05），且其蛋白表达与临床分期及病理分级有关（P〈0．05），而与患者年龄、腹水细胞学、淋巴结转移无关（P〉0．05）。结论：B7-H4在卵巢浆液性癌的高表达及其与临床分级分期的关系，提示其可能与肿瘤发生发展有关，可为卵巢恶性肿瘤诊断及治疗提供靶位点。Objective： To evaluate the expression of B7-H4 in epithelial ovarian serous carcinoma and the relationship between B7-H4 expression and the clinicopathological variables. Methods i Archival tissue blocks from 74 epithelial ovarian tumor patients including 6 benign tumors, 10 borderline tumors ,41 serous carcinomas ,5 endometrioid carciniomas,2 clear cell carcinomas and 10 mucinous carcinoma were evaluated by RT-PCR for the expression of B7-H4 mRNA and immunohistochemistry for the expression of B7-H4 protein . The comparations were done between the clinicopathological variables and B7-H4 expression in 41 ovarian serous carcinoma tissue blocks. Results：B7-H4 mRNA were detected in all above cases including benign, borderline and malignant ovarian tumors. The positive proportions of B7-H4 protein in benign, borderline and malignant epithelial ovarian tumors were 2/6,6/10 and 52/58, which showed malignant cases were significantly higher than nonmalignant ones （P 〈 0.05）. The positive proportion of B7-H4 protein in serous ,endometrioid ,clean cell and mucinous ovarian carcinomas were 41/41,5/5,2/2 and 4/10,which showed the first three were significantly higher than last one （P 〈0.05）. There was no significant difference in the distribution of the proportion of B7-H4 positive cells in serous carcinoma in the four groups including 〈 10%, 〉 10% - 50%, 〉 50% - 80%, 〉 80% - 100% （P 〉0.05）. The expression of B7-H4 protein in 41 ovarian serous carcinomas revealed that there was no correlation between B7-H4 expression and age, ascites cytology or lymph node metastasis （P 〉 0.05 ） except for stage and grade （ P 〈 0.05 ）. Conclusions： The up-regulated expression of B7-H4 in ovarian serous carcinoma which has positive relationship with later stage and lower grade revealed that B7-H4 plays an important role in cancer generation and development, which provided a potential target for the therapy and diagnosis of ovarian carcinoma.

关 键 词：卵巢肿瘤 B7抗原 B7-H4 蛋白表达 病理学 临床 

分 类 号：R737.31[医药卫生—肿瘤]