沙利度胺治疗脉络膜新生血管的实验研究  

Effects of thalidomide on experimental choroidal neovascularization of mouse

作　　者：马敏旺[1] 陈松[1] 李文博[1] 

机构地区：[1]天津市眼科医院,武警医学院附属医院300020

出　　处：《临床眼科杂志》2008年第4期360-362,共3页Journal of Clinical Ophthalmology

基　　金：天津市科技发展计划资助项目(33111411)

摘　　要：目的探讨沙利度胺(thalidomide)对脉络膜新生血管(CNV)的抑制作用及毒副作用。方法健康C57BL/6J小鼠采用氪红激光光凝建立CNV模型。随机分组:A组为对照组;B组腹腔注射沙利度胺,浓度为10mg/kg;C组腹腔注射沙利度胺浓度为50mg/kg。连续应用10d。记录实验前后小鼠体重变化。从防御反射、捕捉难易度、进食量、皮毛颜色及精神状态等5个方面来评估小鼠行为学改变。病理组织学检查测量CNV组织厚度。结果小鼠体重变化有明显差异,C组小鼠体重增加明显小于A组和B组(P<0.01)。A组和B组小鼠行为学基本没有改变。C组小鼠行为学改变明显。3组小鼠CNV组织中央厚度有明显差异;A组小鼠CNV组织中央厚度高于B、C两组(P<0.01)。结论沙利度胺能够抑制实验性CNV;在常规用药剂量下,小鼠能较好的耐受沙利度胺,当药物浓度过大时,其抑制CNV的作用没有增加,但副作用明显严重。Objective To determine if thalidomide can inhibit Choroidal Neovascularization （CNV） and evaluate the toxicity of thalidomide. Methods CNVmodel was established by Krypton laser in C57BL/6J mice. Group A： the control group, without thalidomide. Group B： with 10mg/kg thalidomide through abdominal cavity injection. Group C： with 50mg/kg thalidomide through abdominal cavity injection. Record and evaluate the change of mouse＇ s body weight., defense reflex the difficulty of capture, food intake, color of hair and mental state. The thickness of CNV complex was measured and analysed. Results There was significant difference in the mice of weight change, the change of Group C was less than Group A and B. In pathergasiology, there were no change between Group A and B, but the mouse of Group C have had violent defense reflex, the less food intake and sunken. The CNV thickness of Group A was thicker than Group B and C, the difference was significant, but there was no difference between Group B and C. Conclusion Thalidomide effectively inhibited CNV progression in a mouse model of laser-induced CNV, Mouse can tolerance routine dosage of thalidomide.

关 键 词：沙利度胺 脉络膜新生血管 毒副作用 

分 类 号：R773.4[医药卫生—眼科]