离子交换树脂分离发酵液中乳链菌肽工艺  

SEPARATION OF NISIN FROM FERMENTATION BROTH BY ANIONIC RESIN

作　　者：胡滨[1] 王晖[1] 赵艳丽[1] 殷昊[1] 吴兆亮[1] 

机构地区：[1]河北工业大学生物工程系,天津300130

出　　处：《离子交换与吸附》2008年第4期313-319,共7页Ion Exchange and Adsorption

基　　金：国家中小企业创新基金(05C26111200216)

摘　　要：用阳离子交换树脂D113吸附发酵液中乳链菌肽，单位体积D113的吸附量为120000IU／mL。加入90mL5％KCl溶液，8h时解吸率达到最大值，最终解吸率为48％。在25℃，40℃和50℃下D113的吸附等温线符合Langmuir吸附等温方程。通过对控制机理的判断，25＊（2时F与t呈线性关系，为液膜扩散控制（FDC）；40℃、50℃时[1-3（1-F）^2/3＋2（1-F）-1曲线呈良好的线性关系，为颗粒扩散控制（PDC）。Cation exchange resin D113 was chosen to adsorb nisin from the fermentation broth. The adsorption capacity of D113 was 120000IU/mL. The desorption rate was 48% by using 90mL 5% KCl as desorbent for 8h. At 25℃, 40℃ and 50℃ the absorption of Dl13 could be described by Langmuir isotherm. By judgment of controlling mechanism, at 25℃, there was a well linear relationship between F and t and it was controlled by film diffusion control （FDC）. At 40℃ and 50℃ there was a well linear relationship between [1-3（1-F）^2/3＋2（1-F）] and t and it was controlled by particle diffusion control （PDC）.

关 键 词：乳链菌肽 吸附 解吸 吸附等温线 控制机理 

分 类 号：TQ028.3[化学工程]