酶解和冻融辅助超声提取光合细菌中辅酶Q10的研究  

Study of Extracting CoQ10 from Photosynthetic Bacteria by Ultrasonic Assisted with Zymolysis or Freezing-Thawing

作　　者：许芳[1] 张淑荣[1] 张鹏[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《化学与生物工程》2008年第8期43-45,共3页Chemistry & Bioengineering

摘　　要：研究了酶解及冻融辅助超声提取光合细菌中辅酶Q10的最佳工艺条件,建立了辅酶Q10高产光合菌株的快速筛选方法。结果表明,超声可有效破碎细胞,以提取胞内辅酶Q10,而结合酶解及冻融可以达到更好的效果,在优化条件下,辅酶Q10的提取率分别提高5.1%和9.4%。The optimum extraction process of coenzyme Q10 from photosynthetic bacteria by ultrasonic as-sisted with zymolysis or freezing-thawing were studied, then the rapid screening method for photosynthetic bacterium producing high-yield coenzyme Q10 was established. The results showed that, ultrasonic could break the cell efficiently to extract the intracellular coenzyme Q10, and when it was assisted with zymolysis or freezing-thawing,the extraction rate would be improved by 5.l% and 9.4% under optimal conditions.

关 键 词：辅酶Q10 超声破碎 酶解 冻融 

分 类 号：Q814.1[生物学—生物工程]