细胞周期素依赖性激酶2及p27^(Kipl)与血管瘤  

Cyclin-dependent kinase 2 and p27^(Kip1) with hemangiomas

作　　者：杨虹[1] 邓成国[1] 朱代惠[2] 郭青平[1] 

机构地区：[1]郧阳医学院组织学与胚胎学教研室 [2]十堰市房县人民医院,十堰442000

出　　处：《解剖学杂志》2008年第4期483-485,510,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30271345);湖北省科技厅资助(2003ABA164);湖北省卫生厅资助(JXIB075);湖北省科技厅资助(2004AA301c107)

摘　　要：目的:进一步探讨细胞周期素依赖性激酶2(CDK2)及p27Kip1在血管瘤发生、发展及退化过程中的作用机制。方法:采用免疫组织化学SP法检测49例皮肤毛细血管瘤增生期、退化期及正常皮肤组织中CDK2和p27Kip1的表达水平;采用HPIAS-1000高清晰度彩色病理图文报告管理系统,对CDK2和p27Kip1表达的平均光密度和阳性面积率进行图像分析。结果:增生期组CDK2的表达明显高于退化期组和正常皮肤组织组;增生期血管瘤内皮细胞p27Kip1的表达显著低于退化期血管瘤内皮细胞。结论:p27Kip1能抑制血管瘤内皮细胞的增殖,在血管瘤的退化过程中起了重要作用;而CDK2能促进血管瘤内皮细胞增殖,在血管瘤的增生过程中起了重要作用。Objective： To investigate the role of CDK2 and p27^kip1 in the pathogenesis, development and degeneration of human hemangiomas. Methods.. The expressions of CDK2 and p27^kip1 were detected in proliferative, degenerative hemangiomas and normal skin tissues by immunohistochemical technique. Average optical density and positive area rate of the expression of CDK2 and p27^kip1 proteins were measured by image analysis （HPIAS-100）. Results.. The expression of CDK2 in prolifera- tive hemangiomas was significantly higher than that in degenerative hemangiomas and normal skin tissues. The expression of p27^kip1 in proliferative hemangiomas was significantly lower than that in degenerative hemangiomas. Conclusion： It is suggested that CDK2 might progress the proliferation of endothelial cells in human proliferative hemangiomas; while p27^kip1 might inhibit the proliferation of endothelial cells in involuting hemangiomas.

关 键 词：血管瘤 细胞周期素依赖性激酶2 P27^KIP1 免疫组织化学 

分 类 号：R732.2[医药卫生—肿瘤]