小细胞肺癌细胞SSTR2的体外分布及受体显像  

In vitro distribution and receptor scintigraphy of somatostatin receptor 2 subtypes in small cell lung cancer

作　　者：武凤玉[1] 左书耀[1] 

机构地区：[1]青岛大学医学院附属医院核医学科,青岛266003

出　　处：《肿瘤》2008年第8期672-675,共4页Tumor

基　　金：青岛市科技局科研基金资助项目2004科技发展指导计划(编号:KZD-13)

摘　　要：目的:探讨生长抑素受体亚型2(somatostatin receptor 2 subtype,SSTR2)在体外培养的小细胞肺癌(small cell lung cancer,SCLC)NCI-H446细胞中的表达和分布,以及99mTc-octreotide受体显像对SCLC的诊断价值。方法:应用电子显微镜放射自显影(electron microscope radioautography,EMR)技术,采用125I-octreotide观察SSTR2在SCLC细胞中的分布与表达;自荷人NCI-H446 SCLC裸鼠尾静脉注射0.15mL、活度为16.8MBq的99mTc-octreotide,观察裸瘤鼠模型99mTc-octreotide受体显像的变化。结果:电子显微镜下30 min及120 min组的细胞银颗粒标记率分别为95%(19/20)和80.0%(16/20),2者比较差异有统计学意义(P<0.05);30 min组银颗粒主要分布于细胞膜区,120 min组银颗粒主要分布于细胞质和细胞核区。过量加入Tyr-3-octreotide的对照组中银颗粒数明显少于其对应的未加过量组。5例荷瘤裸鼠的受体显像均呈阳性,且在注射99mTc-oct-reotide 4 h后肿瘤显像最清晰。结论:SCLC肿瘤细胞中高表达SSTR2,octreotide受体显像可能成为一种新的早期探测SCLC的方法。Objective：To investigate the distribution of somatostatin receptor 2 subtype （SSTR2） in small cell lung cancer （SCLC） in vitro and the value of ^99mTc-octreotide scintigraphy for SCLC diagnosis in vivo. Methods ：The distribution of SSTR2 was detected by electron microscopic autoradiography （EMR） using ^125I octreotide. ^99mTc-octreotide （0.15 mL, 16.8 MBq） was injected into nude mice via tail veins and ^99mTc-octreotide scintigraphy was observed. Results：The tagged rates of cellular sliver grains were 95.0% （19/20） and 85.0% （16/20） at 30 min and 120 min, respectively. The difference was significant （ P 〈0.05）. Sliver grains were distributed in the membranes at 30 min and located in the nucleolus and cytoplasm at 120 min. The numbers of sliver grains in the control group （addition of over Tyr 3-octreotide） were remarkably less than those of group 30 min and 120 min. The scintigraphy of the tumors in 5 nude mice was positively displayed at 4 h postinjection of ^99mTc octreotide. Conclusions：SSTR2 is over-expressed in SCLC. Radiolabeled octreotide scintigraphy may become a novel detection method for early diagnosis of SCLC.

关 键 词：癌 小细胞 受体 生长抑素 放射自显影术 

分 类 号：R734.2[医药卫生—肿瘤]