高效菲降解菌降解特性与蛋白组学研究  

Degrading Characteristics and Proteome of Phenanthrene-degrading Bacterium

作　　者：高林[1] 肖明[1] 郭鲁申[1] 陆贻通[2] 赵渝[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海交通大学资源与环境系,上海201101

出　　处：《应用与环境生物学报》2008年第4期523-527,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No. 20337010);国家基础研究重大项目(No. 2004CB41850);上海师范大学校基金资助(No. DKL835)~~

摘　　要：研究了各种条件下高效菲降解菌ZX16对菲的降解特性,并借助一维与二维蛋白质组学分析技术,比对了菲诱导前后ZX16蛋白表达差异.结果表明,该菌株利用菲生长的最适温度为35℃,最适pH为7.5,可以耐受并降解2500mgL-1菲,不能利用邻苯二甲酸,但可利用水杨酸和邻苯二酚.菌株细胞蛋白SDS-PAGE结果显示,菌株在菲诱导12h后,出现两条Mr为19×103和27×103的新增蛋白条带,从二维电泳图谱的相应分子量位置上发现3个新增表达蛋白,质谱分析与生物信息学检索结果表明,2个点分别为芳香族加氧酶小亚基,1个点为顺式氯苯二氢二醇脱氢酶.The degrading characteristics and the enzymes induced during the phenanthrene degradation by the phenanthrene-degrading bacterium ZX16 were investigated. The experiments of degrading characteristics showed that the strain was able to utilize phenanthrene as carbon and energy sources with the maximum tolerance concentration of phenanthrene 2 500 mg/L. The optimum temperature and pH for phenanthrene degradation by ZX16 were 35℃ and 7.5, respectively. ZX16 was able to utilize salicylic acid and catechol instead of phthalate. As a means to identify phenanthrene-induced proteins, the protein extracts from the bacteria grown on phenanthrene were analyzed with one-dimensional gel and two-dimensional gel electrophoresis. Whole cell protein SDS-PAGE map of the strain after growing for 12 h on phenanthrene showed that two new brands with Ms of about 18×10^3 and 27×10^3 appeared. Three distinctive protein spots, which were observed only in induced cells but not found in non-induced cells, were further analyzed by MALDI-TOF after 2D-PAGE. Two spots showed positive identification of small subunit aromatic oxygenase, and one spot indicated positive identification of cis-chlorobenzene dihydrodiol dehydrogenase. Fig 5, Tab 2, Ref 20

关 键 词：菲 多环芳烃 降解特性 蛋白质组学 

分 类 号：X172[环境科学与工程—环境科学] Q78[生物学—分子生物学]