外源性FHIT基因对人胶质瘤细胞U87增殖与凋亡的影响  

Effects of exogenous FHIT gene on proliferation and apoptosis of human glioma cell line U87

作　　者：夏养华[1] 沈建康[1] 

机构地区：[1]上海交通大学医学院瑞金医院神经外科,上海200025

出　　处：《上海交通大学学报（医学版）》2008年第8期1000-1002,共3页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨外源性脆性组氨酸三联体(FHIT)基因的表达对胶质瘤细胞系U87增殖和凋亡作用的影响。方法通过脂质体介导将载有人外源性FHIT基因的真核表达质粒pcDNA3.1/myc-His(-)B-FHIT和空载体质粒pcDNA3.1/myc-His(-)B分别转染胶质瘤细胞系U87。实验细胞分U87-FHIT组(转染FHIT基因的U87细胞)、U87-vector组(转染空载体质粒的U87细胞)和空白对照组(亲本U87细胞)。Western blot和免疫荧光染色检测外源性FHIT蛋白的表达;MTT法及流式细胞术观察转染前后细胞生长特性的变化。结果U87-FHIT组细胞的生长抑制率和凋亡率明显高于U87-vector组和空白对照组,差异均有统计学意义(P<0.05)。结论外源性FHIT基因表达能诱导U87细胞凋亡,抑制其生长,具有抗胶质瘤作用。Objective To investigate the effects of exogenous fragile histidine triad（FHIT） gene on apoptosis of human glioma cell line U87. Methods By the method of liposome transfection,plasmids pcDNA3.1/myc-His（-）B-FHIT and pcDNA3.1/myc-His（-）B were transfected into glioma cell line U87.U87 cells were divided into three groups： U87-FHIT group,U87 cells transfected by plasmids pcDNA3.1/myc-His（-）B-FHIT;U87-vector group,U87 cells transfected by plasmids pcDNA3.1/myc-His（-）B;and blank control group,U87 cells without transfection.The expression of exogenous FHIT protein was detected by Western blot and immunofluorescence staining.The effects of FHIT on the growth characteristics of U87 were observed by MTT and flow cytometry. Results Growth inhibitory rate and apoptosis rate of the cells in U87-FHIT group were significantly higher than those in U87-vector group and blank control group（P〈0.05）.Conclusion The expression of exogenous FHIT gene can induce apoptosis and inhibit growth of U87 cells,thereby have the anti-glioma effect.

关 键 词：脆性组氨酸三联体基因 胶质瘤 基因转染 细胞凋亡 

分 类 号：Q78[生物学—分子生物学] R739.41[医药卫生—肿瘤]