对南美白对虾WSBV病毒的定性和定量检测研究  

Research of Qualitative and Quatitative PCR Detection Technology of White Spot Bacilliform Virus

作　　者：曹新志 王柱[1] 谢溢[2] 

机构地区：[1]四川理工学院食品与生物技术应用研究所,四川自贡64300 [2]成都中医药大学峨眉学院,四川峨眉614201

出　　处：《四川理工学院学报（自然科学版）》2008年第4期58-60,64,共4页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

摘　　要：利用PCR定性和定量检测对100尾南美白对虾做了研究,发现致病虾在定性检测中成阳性,健康虾成阴性或者弱阳性;致病虾在定量检测中WSBV含量大于103个病毒粒子/mg,健康虾小于等于103个病毒粒子/mg,由此可以得知白斑病爆发的临界值为103个病毒粒子/mg。In this paper, 100 shrimps are determi ned by qualitative and quantitative PCR. The result shows that shrimps with obvious symptom are mescaline gender in the qualitative examination and shrimps without symptom are negative or weak mescaline gender; shrimps with obvious symptom carry more than 103 virus particles per microgram tissue, while shrimps with obvious symptom carry less than the amount. So 103 virus particles per microgram tissue would be the critical point for the shrimp virus disease outbreak.

关 键 词：南美白对虾 WSBV PCR 

分 类 号：Q179.2[生物学—水生生物学]