黑曲霉菌丝体真空冷冻干燥的研究  

Preservation of the Mycelium of Aspergillus Niger by Freeze Drying

作　　者：丁春杰[1] 肖更生[1] 陈卫东[1] 徐玉娟[1] 张友胜[1] 吴继军[1] 金盛楠[1] 

机构地区：[1]广东省农业科学院蚕业与农产品加工研究所

出　　处：《现代食品科技》2008年第8期745-747,834,共4页Modern Food Science and Technology

基　　金：国家科技支撑计划(2006BAD05A02-01);2006年省部产学研结合项目(2006D90204006)资助

摘　　要：本文研究了黑曲霉(ATCC16404)菌丝体真空冷冻干燥的保藏方法,并从培养基和保护剂对冻干菌的影响进行了研究,初步确定了高活菌数的增殖培养基。比较了不同保护剂对菌丝体成活率的影响,确定了最佳保护剂,活菌数达到109cfu/g。In this paper, the preservation of the mycelium of Aspergillus niger by freeze drying was introduced ,and the effects of the cultural medium and freeze-drying protectant were investigated. The enrichment culture medium for Aspergillus niger with high viable cells number and the best freeze-drying protectant were determined, with which the viable cells number of Aspergillus niger treated by freeze drying were 10^9cfu/g.

关 键 词：黑曲霉菌丝体 增殖培养基 冷冻干燥 保护剂 

分 类 号：Q93-3[生物学—微生物学]