一种适用于大规模转基因作物PCR检测的简易DNA提取方法  

Study of a Simplified DNA Extraction Method for the Large-scale PCR Detection of Genetically Modified Crops

作　　者：钟罗宝[1] 陈谷[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《现代食品科技》2008年第8期794-797,815,共5页Modern Food Science and Technology

摘　　要：近年来,转基因农作物的数量和种类大幅度增加,其安全性一直受到各国政府的重视和关注,转基因食品的检测也是各国贸易壁垒的一个重要方面。PCR技术作为一种快速、准确的核酸检验方法,广泛地应用于转基因农作物和食品的检测中。DNA样品的制备速度和质量是PCR检测方法的限速步骤之一,本文改进了碱裂法提取植物DNA,并与SDS快速提取法及CTAB法作比较,用于不同长度片段的PCR扩增。结果显示:利用改进后的碱裂解法提取的DNA为模板可以稳定扩增出1000bp以内的片段,这是一种简易的适合于大规模转基因作物和食品PCR检测的DNA提取方法。Recently, with the increase of quantity and species of transgeuic crops, more and more attention has been paid on their safety. As a rapid and accurate method to detect the nucleic acids, PCR is widely used in the detection of genetically modified （GM） crops and food and one of its rote-limiting steps is the speed and quality of DNA extraction. Here the alkaline lysis method is modified and compared with SDS and CTAB methods in PCR amplification of fragments with different lengths. The results show that fragments less than 1000 bp can be steadily amplified from the DNA exlracted by the modified alkaline lysis method. It is confirmed that the modified alkaline lysis method is suitable to be applied in the large-scale and rapid PCR detection of genetically modified crops as well as GM food.

关 键 词：转基因农作物 PCR检测 DNA提取 碱裂解法 拟南芥 

分 类 号：S188[农业科学—农业基础科学]