骨肉瘤中基质金属蛋白酶-9、血管内皮生长因子表达的意义  

Expressions and implication of MMP-9 and VEGF in osteosarcoma

作　　者：黄自锋[1] 杨述华[1] 刘勇[1] 郑东[1] 李建莎[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022 [2]华中科技大学同济医学院病理教研室,武汉430030

出　　处：《华中医学杂志》2008年第4期247-248,256,共3页Central China Medical Journal

摘　　要：目的探讨基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)的表达与骨肉瘤血管新生及临床复发转移的关系。方法采用免疫组织化学法检测46例骨肉瘤标本MMP-9、VEGF、CD34表达。结果MMP-9、VEGF的阳性表达率分别为76.09%、65.22%。不同VEGF表达组间MMP-9、MVD表达有显著性差异,MVD与MMP-9、VEGF表达强度呈正相关(P<0.05)。转移组与复发组间VEGF表达有显著性差异(P<0.05)。结论MMP-9、VEGF共同参与骨肉瘤的血管生成并协同表达。检测VEGF和MMP-9表达与MVD值可作为判断骨肿瘤预后的重要指标。Objective To investigate the expressions of MMP-9 and VEGF and their relationships to the recurrence and metastasis of osteosarcoma. Methods The expressions of MMP-9, VEGF and CD34 in 46 cases of osteosarcomas were detected by using immunohistochemical staining and intratumoral microvessel density（MVD） was calculated. Results The expression of MMP-9 and VEGF in osteosarcoma was 76. 09% and 65.22 % respectively, and the VEGF expressions was significantly related to the invasion and recurrence of the tumor（P〈0, 05）. There was significant difference in the expression of MMP-9 and MVD between VEGF positive and negative groups, and the expressions of VEGF and MMP-9 was significantly associated with MVD （PM〈0. 05）. Conclusion The expressions of VEGF were highly related to the recurrence and metastasis of osteosarcoma, MMP-9 and VEGF stimulate angiogenesis of osteosarcoma and are co-expressed. Detection of the MMP-9 and VEGF expression and MVD may be useful for evaluating theprognosis of malignant bone tumors.

关 键 词：骨肉瘤 MMP-9蛋白 血管内皮生长因子 微血管密度 

分 类 号：R738.1[医药卫生—肿瘤]