十五碳二元酸发酵过程中β氧化的控制  

Inhibition of β oxidation on Pentadecane-dioic Acid Fermentation

作　　者：王丽菊[1] 余晓斌[2] 

机构地区：[1]江南大学生物工程学院 [2]工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《生物技术》2008年第4期76-78,共3页Biotechnology

摘　　要：目的:提高烃酸转化率,降低发酵法生产十五碳二元酸成本。方法:考察几种碳源和β氧化抑制剂丙烯酸对菌体生长、烃酸转化率及产酸的影响,并对乙酸钠的作用机制进行讨论。结果:发酵培养基加入的几种碳源中,乙酸钠对十五碳二元酸发酵影响最大。加入0.4(W/V)乙酸钠,转化率比对照组提高21%,产酸量比对照组提高22.5%;转入产酸期后加入0.1(V/V)丙烯酸,产酸进一步提高16%。结论:乙酸钠和丙烯酸能够部分控制β氧化,可以有效降低发酵法生产十五碳二元酸成本。Objective： To improve conversion of pentadecane to pentadecane - dioic acid（DC15 ）, and reduce the production cost DC15 by ferment- ing. Method： Studies on effects of several kinds of carbon sources and β oxidation inhibitor- acrylic acid on the fermentaion of DC15. Cell growth, conversion of pentadecane and DC15 yield were herein considered. The mechan/sm of sodium acetate was also discussed. Result： The con- version of pentadecane improve 21% after adding 0.4（W/V） sodium acetate ,DC15 yield was increased 22.5% accordingly,the yield was further improved 16% after adding 0. 1（V/V） acrylic acid on the period of acid produce. Conclusion： Sodium acetate and acrylic acid can inhibit β oxidation and reduce the production cost.

关 键 词：β氧化 十五碳二元酸 乙酸钠 丙烯酸 

分 类 号：TQ921[轻工技术与工程—发酵工程] TQ332.5