小鼠孕早期Cyclin-E表达规律及意义  

Expression and significance of cyclin-E in the mouse endometrium during the early pregnancy

作　　者：张谭[1] 周生健 魏莎莉[1] 杨戎[1] 孟君[1] 马海兰[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生理研究室,重庆400016 [2]重庆市渝中区人口和计划生育生殖健康中心,重庆400010

出　　处：《重庆医科大学学报》2008年第8期954-958,共5页Journal of Chongqing Medical University

摘　　要：目的:探讨细胞周期素E(Cyclin-E)在早孕小鼠子宫内膜的的表达及其对小鼠胚胎着床数的影响。方法:应用RT-PCR及免疫组织化学的方法检测早孕小鼠子宫内膜细胞Cyclin-E基因和蛋白的表达规律;观察子宫角注射抗Cyclin-E抗体后对小鼠胚胎着床数的影响。结果:妊娠第1～7d小鼠子宫内膜组织均有Cyclin-E表达,自妊娠2d起表达增强,5d表达量达高峰。小鼠妊娠期Cyclin-E在子宫内膜腔上皮、腺上皮及内膜基质中均有表达。单侧子宫抗Cyclin-E抗体注射后双侧胚胎着床数差值较对照组(注射等体积生理盐水)明显增大。结论:在小鼠胚胎着床过程中,Cyclin-E可能参与调节子宫内膜的增殖分化,在子宫内膜蜕膜化及胚胎着床过程发挥作用。Objective：To investigate the expression rule of Cyclin-E in endometrium during the early pregnancy and its role in blastocyst implantation in mice. Methods：RT-PCR and immunohistochemistry techniques were applied to detect Cyclin-E gene and protein expression in endometrium from pregnant mice on days 1 - 7. In addition Anti-cyclinE antibody was injected into horns of uterus of day 3 pregnant mice to observe the numbers of implanted blastocysts. Results：Expression of Cyclin-E gene and protein in pregnant mice were steady increasing since day 2 and reaching maximal level on day 5 of pregnancy. Injection of Anti-cyclinE antibody could decrease the numbers of implanted blastocyst compared with that of saline-injected group. Conclusion：Cyclin-E were persistently expressed in mouse endometrium during the early pregnancy and might be concerned with proliferation differentiation of endometrium and participates in the regulation process of mouse blastodyst implantation.

关 键 词：细胞周期素E(Cvclin—E) 着床 子宫内膜 RT—PCR 免疫组织化学 

分 类 号：R394[医药卫生—医学遗传学]