粉防己碱对喉癌耐药细胞株KBV200耐药逆转作用的研究  

Reversal effect of tetrandrine on multidrug resistance of the KBV200 cell line

作　　者：叶琳[1] 陈鸿雁[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016

出　　处：《重庆医科大学学报》2008年第8期967-969,共3页Journal of Chongqing Medical University

摘　　要：目的:研究天然有效成分粉防己碱(Tetrandrine,TET)对喉癌耐药细胞株KBV200的耐药逆转作用。方法:本研究以喉癌耐药细胞株KBV200为研究对象,采用粉防己碱逆转KBV200对常用抗肿瘤药物阿霉素的多药耐药作用,以经典逆转剂维拉帕米(Verapamil,VRP)为对照组,用四甲基偶氮唑蓝(MTT)法测定其对实验细胞的增殖抑制率。结果:低毒剂量的粉防己碱能逆转实验细胞株对阿霉素的多药耐药(Multidrug resistance,MDR)作用,逆转指数为22.96;维拉帕米逆转指数为9.31。结论:低毒浓度的天然药物粉防己碱能逆转喉癌耐药细胞株KBV200对阿霉素的耐药作用,其逆转效果优于经典逆转剂维拉帕米。Objective：To study the reversal effect of tetrandrine on muhidrug resistance of KBV200 cell line in order to provide experimental data for clinical individual chemotherapy. Methods：MTT reduction assay was used to investgate the inhibition of Adriamycin on KBV200cell line,and the reversal effects of Tetrandrine and Verapamil on the cell line. Results：Verapamil and Tetrandrine had the reveral effects on the cell line,and the reversal indexes of Tetrandrine and Veramil were 22.96 and 9.31, respectively. Conclusion：KBV200 cell line was muhidrug resistance to Adriamycin but Tetrandrine had reversal effects on its muhidrug resistance.

关 键 词：粉防己碱 维拉帕米 KBV200细胞系 多药耐药 

分 类 号：R73-354[医药卫生—肿瘤]