氟代柠檬酸对体外培养的神经胶质瘤细胞G422增殖的抑制效应  

Inhibitory Effect of Fluorocitrate on the Proliferation of Cultured Glioblastomas G422 Cells

作　　者：瞿家桂[1,2] 王正波[2] 董锦润[2] 马原野[2,3,4] 

机构地区：[1]中国科学技术大学生命科学学院 [2]中国科学院昆明动物研究所灵长类认知实验室 [3]中国科学院昆明灵长类研究中心,云南昆明650223 [4]昆明Biomed International,云南昆明650223

出　　处：《Zoological Research》2008年第4期427-430,共4页动物学研究（英文）

基　　金：National Science Foundation of China (30470553; 30530270; 30670669; 30770700);973 program (2005CB522803; 2007CB947703);863 program (O7013810; 2006AA02A116);the Major State Basic Research of China (2003CB716600);Chinese-Finnish International Collaboration Project-neuro (30621130076);Program of CASC (KSCX1-YW-R-33; YZ200737);National Key Technologies R&D Program;Yunnan Science and Technique Program (2006PT08-2)

摘　　要：为研究氟代柠檬酸(Fluorocitrate)对体外培养的神经胶质瘤细胞生长的影响,采用MTT法研究不同的氟代柠檬酸浓度(0.0025mmol/L,0.005mmol/L,0.01mmol/L,0.025mmol/L和0.1mmol/L)和作用时间(36h,48h和60h)对神经胶质瘤细胞G422增殖的影响。结果发现:(1)氟代柠檬酸可抑制G422细胞的增殖,并且其抑制作用随氟代柠檬酸浓度的增加而增强;(2)高浓度(0.01mmol/L,0.025mmol/L和0.1mmol/L)氟代柠檬酸对G422细胞的增殖抑制作用随作用时间的延长而增强;(3)低浓度(0.0025mmol/L和0.005mmol/L)氟代柠檬酸对G422细胞的增殖抑制作用不随作用时间的延长而改变。实验表明,氟代柠檬酸能够抑制神经胶质瘤细胞的增殖,其抑制能力随氟代柠檬酸浓度的增加和作用时间的延长而加强。In the present study, the effects of fluorocitrate on the proliferation of cultured glioblastomas G422 cells were investigated. Proliferation of glioblastomas G422 cells was measured by MTT assay at 36h, 48h and 60h after fluorocitrate （0.0025mmol/L, 0.005mmol/L, 0.01 mmol/L, 0.025mmol/L and 0.1 mmol/L） treatment. Our results showed that： （1） Fluorocitrate inhibited the proliferation of glioblastomas G422 cells in a dose dependent manner; （2） The inhibition rate increased with treatment time at high concentrations of fluorocitrate （0.01 mmol/L, 0.025mmol/L and 0.1 mmol/L）; （3） The inhibition rate did not increase with treatment time at low concentrations of fluorocitrate （0.0025 mmol/L and 0.005mmol/L）. This study indicated that fluorocitrate inhibited the proliferation of glioma cells as a function of fluorocitrate concentration and treatment time.

关 键 词：氟代柠檬酸 神经胶质瘤细胞 MTT 增殖 抑制效应 

分 类 号：R739.4[医药卫生—肿瘤]