人脑星型胶质母细胞瘤裸小鼠术后转移模型的建立  

Establishment of Postoperative Metastatic Animal Model of Human Glioblastoma in Nude Mice

作　　者：闫明霞[1] 刘蕾[1] 荚德水[1] 吴海燕[1] 王晓敏[1] 孔韩卫[1] 姚明[1] 

机构地区：[1]上海市肿瘤研究所实验病理研究室,上海200032

出　　处：《实验动物与比较医学》2008年第4期204-208,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科学技术委员会"登山行动计划"项目(编号:064909001)

摘　　要：目的建立人脑星型胶质母细胞瘤裸小鼠术后转移模型,为脑胶质瘤的基础和临床研究提供理想的实验动物模型。方法首先建立27只裸小鼠人脑星型胶质母细胞瘤U87MG皮下移植瘤模型,待肿瘤长至20 mm左右时18只裸小鼠手术切除皮下肿瘤建立术后转移动物模型,其余9只作为对照组,动物处于濒死状态时进行大体解剖观察远处脏器转移,并观察组织病理学改变。结果成功建立了人脑星型胶质母细胞瘤术后转移模型,动物的平均寿命约67.3 d,肺表面可见若干个大小不等的透明结节,平均肺重为0.83±0.30 g,肺转移率达100%(18/18),胸腔淋巴结转移率达66.67%(12/18),1只动物出现心内转移。对照组动物的平均荷瘤寿命为56 d,1只动物出现肺转移,肺转移率为11.11%(1/9),平均肺重为0.41±0.12 g。结论本模型较好地模拟了临床肿瘤根除术后发生自发远处转移的过程,为脑胶质瘤的转移机理及抗转移治疗等相关实验研究提供了理想的动物模型。Objective To establish a metastatic animal model of human glioblastoma by surgical tumor resection in nude mice, so as to provide an ideal animal model for experimental and clinical studies. Methods Histological intact tumor lesions of human glioblastoma U87MG were transplanted subcutaneously into twenty-seven nude mice. When the tumor reached 20 mm in diameter, eighteen of these nude mice were randomly selected as experimental group and had their tumors resected to establish the postoperative metastatic animal model. The rest were assigned to the control group. All mice were sacrificed when the animals were obviously slender, the tumor metastasis rates and pathological changes were observed. Results The postoperative metastatic animal model of human glioblastoma were successfully established. In the experimental group, the average survival time of nude mice was 67.3 days, the average lung weight was 0.83±0.30 g, the metastasis rates in lung, lymph node, and cardia was 100% （18/18）, 66.67% （12/18） and 5.56%（1/18）, respectively. While in the control group, the average survival time of nude mice was 56 days, the metastasis rate of lung was 11.11% （1/9）, the average lung weight was 0.41±0.12 g. Conclusions This animal model simulates the metastasis after clinical operation, it is an ideal animal model for pathogenesis exploration and anti-metastasis treatment of human glioblastoma.

关 键 词：人脑星型胶质母细胞瘤 转移 疾病动物模型 手术切除 

分 类 号：R739.41[医药卫生—肿瘤]