PTEN,P-AKT,P-ERK蛋白在胃癌组织中的表达及意义  

Expression of PTEN,P-AKT,P-ERK protein in gastric carcinoma tissues and their clinical significance

作　　者：王兵[1] 周士福[1] 蔡凤林[1] 马兆生[1] 

机构地区：[1]无锡市第四人民医院普外科,江苏无锡214062

出　　处：《现代肿瘤医学》2008年第9期1568-1570,共3页Journal of Modern Oncology

摘　　要：目的:检测PTEN和P-AKT,P-ERK蛋白在胃癌中的表达,并探讨其相互关系。方法:应用免疫组织化学方法检测65例胃癌及癌旁组织中PTEN和P-AKT,P-ERK蛋白的表达情况。结果:PTEN蛋白在胃癌中表达的阳性率(58.46%)明显低于相应正常组织(100%)(P<0.01),其表达水平与组织分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05)。P-AKT蛋白在胃癌中表达的阳性率(67.69%)明显高于相应正常组织(26.15%)(P<0.01),其表达与淋巴结转移有关(P<0.01),与浸润深度、分化程度和TNM分期无关(P>0.05)。P-ERK蛋白在胃癌中表达的阳性率(86.15%)明显高于相应正常组织(16.92%)(P<0.01),其表达水平与浸润深度、淋巴结转移及TNM分期有关(P<0.05),与分化程度无关(P>0.05)。胃癌组织中PTEN和P-AKT、PTEN和P-ERK蛋白表达之间呈明显负相关(P<0.01)。结论:PTEN的低表达或失表达,可能与P-AKT、P-ERK的异常激活有一定联系,从而对胃癌的发生、发展起重要作用。Objective：To investigate the expression of PTEN, P- AKT and P- ERK protein in gastric carcinoma tissues and relationship between their expression and clinicalpathology parameters. Methods： Immunohistochemical assay was used to detect expressions of PTEN, P - AKT and P - ERK in 65 gastric cancer and adjacent tissues. Resuits：The positive rate of PTEN was significantly lower in gastric cancer （58.46%） than that in control tissues （ 100% ） （ P 〈 0.01 ） , and expression level of PTEN was related to histological differentiation, depth of invasion, lymphnode metatasis and TNM stage （ P 〈 0. 05 ）. The positive rate of P - AKT was significantly higher in gastric cancer（67.69% ） than that in control tissues （26.15%） （ P 〈 0.01 ）, and expression level of P - AKT was significantly correlated with lymphnode metatasis（ P 〈 0.01 ） ;no correlation was observed with histological differentiation, depth of invasion and TNM stage（ P 〉 0.05 ）. The positive rate of PERK was significantly higher in gastric cancer （86.15%） than that in normal tissues（ 16.92% ）, and expression level of P -ERK was related to depth of invasion, lymphnode metatasis and TNM stage （ P 〈 0.05 ） ; no correlation was found between the expression level of P - ERK and histological differentiation（ P 〉 0.05 ）. PTEN was correlated negatively with P - AKT（ P 〈 0.01 ） and P - ERK （ P 〈 0.01 ） expression. Conclusion：The decrease or deletion of PTEN expression may be related to the abnormal activation of P- AKT and P- ERK protein,and play an important role in the initiation and development of human gastric cancer.

关 键 词：胃癌 PTEN P—AKT P—ERK 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]